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Detection of NPM-ALK DNA rearrangement in CD30 positive anaplastic large cell lymphoma |
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| Authors: | W. Waggott Y.-M. D. Lo C. Bastard K. C. Gatter D. Leroux D. Y. Mason J. Boultwood J. S. Wainscoat |
| Affiliation: | Leukaemia Research Fund Molecular and Cytogenetic Haematology Unit;Nuffield Department of Clinical Medicine;Department of Cellular Science, John Radcliffe Hospital, Oxford, U.K.;Department of Cytogenetics, Centre Regional de Transfusion Sanguine, Bois-Guillaume, France;Department of Genetics, University Hospital of Grenoble, France |
| Abstract: | CD30 positive anaplastic large cell lymphoma (ALCL) is a type of non-Hodgkin's lymphoma associated with a specific chromosome translocation between chromosomes 2 and 5. Recent molecular characterization of the translocation breakpoint has identified a gene fusion between NPM (nucleophosmin) and ALK (anaplastic lymphoma kinase). Using a DNA hybridization technique, the NPM rearrangement was found among 5/5 ALCL samples. We have developed a PCT methodology which has enabled the detection of the NPM-ALK rearrangements amongst seven t(2; 5)(p23; q35) ALCL cases based on a long-range PCR of genomic DNA. The rapidity and robustness of this method may have diagnostic applications for ALCL. |
| Keywords: | Ki-l (CD30) positive anaplastic large cell lymphoma NPM-ALK rearrangement t(2 5)(p23 q35) PCR diagnosis long-range PCR |