卵巢肿瘤组织中C-erbB_2、P_(16)基因的表达及与预后的关系

目的:探讨卵巢肿瘤中癌基因C-erbB2及抑癌基因P16的表达及其与临床病理特征及患者预后的关系。方法:用免疫组织化学ABC法,分析98例卵巢恶性肿瘤、24例交界性肿瘤、30例良性肿瘤、10例正常组织石蜡包埋组织中C-erbB2、P16的表达情况。结果:①C-erbB2在卵巢恶性肿瘤、交界性肿瘤、良性肿瘤之间的表达存在显著性差异(P<0.01)。C-erbB2的表达率及表达强度与组织分化程度及生存期有显著相关性(P<0.05),C-erbB2的过度表达见于恶性程度高、组织分化差、预后不良的肿瘤中。②P16在卵巢恶性肿瘤、交界性肿瘤、良性肿瘤之间的表达存在显著性差异(P<0.01)。其表达强度与组织分化程度及临床分期有显著相关性,在恶性程度比较高、组织分化差、较晚期的肿瘤中P16的表达较弱。结论。癌基因C-erbB2及抑癌基因P16在卵巢癌组织中广泛存在,并与其临床病理指标之间有一定的相关性。提示这些基因在卵巢癌的发生、发展中起一定作用,并对患者临床预后的判断及治疗有一定的指导意义。对各基因之间复杂的相互作用、协同作用还有待于进一步研究。

The expression of C-erbB_2、 P_(16)gene in ovarian tumors and the relationship between its and pronosis

Objective: To study the expression patterns of oncogene C-erbB2and suppressor gene P16 in ovarian tumors; To compare these factors expression with clinic opathological features; and to assess their prognostic value. Methods: Using ABC immunohistochemistry method, investigated the expression of C-erbB2and P16gene in paraffin-embeded 98 cases of maligant ovarian tumors, 24 cases of borderline ovarian tumors and 30 cases of benign ovarian tumors. Results: The expression of C-erbB2 between the maligant, borderlin and benign ovarian tumors is significant in statistic (P<0. 01) . The expression rate and espressive intensity of C-erbB2is associated with differentiation of tumor tissue and survival stage of patients (P>0. 05). The high expression of C-erbB2 is found in those tumors who were more malignant and poorer differentiated and shorter survival stage. The expression of P16 between the maligant, borderlin and benign ovarian tumors is significant in statistic (P<0. 01) . The expression rate and expressive intensity of P16 is associated with differentiation of tumor tissue and clinical different time. The expression of P16 is lower in poorer differentiated and later stage tumors. Conclusion: Oncogene C-erbB2and suppressor gene P16 widely exist in tissues of ovarian cancer and is associated with clinic opathological features. They may play an important role in the development and progression of ovarian cancer.