腹水TNF—α,IL—6,IL—8测定及其临床价值

该研究应用单抗夹心酶联免疫吸附试验（ＥＬＩＳＡ）对１１８例腹水患者的腹水ＴＮＦ－α、ＩＬ－６、ＩＬ－８水平进行测定，探讨其对肝硬化腹水并自发性腹膜炎的诊断和鉴别诊断价值。结果肝硬化腹水并自发性腹膜炎（ＳＢＰ）组其腹水三种细在子的均明显高于其它三组，特别是与低蛋白血症腹水组比较有高度显著差异（Ｐ〈０．０１），与肝硬化腹水组及腹膜转移癌腹水组比较有显著性差异（Ｐ〈０．０５），此两组与低蛋白血症腹水组比

PPA RESEARCH ON THE CLINICAL VALUE OF TNF-2, IL-6AND IL-8 ASSAY IN ASCITES

Objective: To explore the diagnostic and differential. diagnostic value of TNF-2, IL-6 and IL-8 in ascites due to cirrhosiscomplicated with spontaneous peritonitis. Methods: In 118 cases, the levels of TNF-2, IL-6 and IL-8 in ascites were measured byMonoantibody-sandwich ELISA. Results: The levels of TNF-2, IL-6 and IL-8 in the group of cirrhosis ascites complicated withspontaneous peritonitis were significantly higher than those in the group of hypoproteinemia (P<0.01), and than those in both thegroup of cirrhosis ascites and the group of ascites due to peritoneal metastatic carcinoma (P<0.05). The levels of these threecytofactors the last two groups were significantly higher than those in the group of hypoproteinemia (P<0.05). There was nosignificant difference of TNF-2, IL-6 and IL-8 between the cirrhosis ascites group and the metastatic carcinoma group (P>0.05).Conclusions: The results suggest that the assay of the levels of TNF-2, IL-6 and IL-8 is of great reference value for the diagnosisand deferential diagnosis of cirrhosis ascites complicated with spontaneous peritonitis, but not a differentiation index of identifyingbenign ascites from malignant ascites.