血管紧张素Ⅱ和Ⅳ对心血管系统的共同调节

肾素血管紧张素系统(RAS)是心血管疾病的主要调节因素,其核心成分血管紧张素Ⅱ(AngⅡ)不仅可以直接收缩血管、调节血压,还与炎症、内皮细胞功能失调、动脉粥样硬化、高血压、心力衰竭等疾病密切相关。以前大多数研究集中于AngⅡ的功能,以此为靶点生产的AT1受体抑制剂及血管紧张素转换酶Ⅰ抑制剂在心血管疾病的临床治疗中发挥了重要的作用。但是近年来的研究发现,RAS其他成员如Ang(1-7)、AngⅣ有着各自独立的生物学效应,这提示RAS是一个远比我们以往认识

MicroRNA Differential Expression of CD4+T Lymphocyte in Patients with Unstable Angina Pectoris

Aim To screen differential microRNA(miRNA) expression profiles of CD4+T lymphocyte from the patients with unstable angina pectoris(UAP) and the healthy controls by microarray analysis technique.To elucidate the mechanism responsible for modulation of CD4+T lymphocyte and provide insights into the effects of miRNA on UAP. Methods The CD4+T lymphocyte were isolated from mononuclear cells prepared with Ficoll-Hypaque density-gradients centrifugation from human peripheral blood by magnetic cell sorting system(MACS).The miRNA expressions profiles of CD4+T lymphocyte were screened using oligonucleotide microarray and differentially expressed miRNA were identified by using microarray analysis software.The microarray results were confirmed by real-time quantitative polymerase chain reaction(qRT-PCR). Results The expression of miR-155,miR-21,miR-424 and miR-127-3p were up-regulated over 1.5 folds,and the expression of miR-30b and miR-181a were down-regulated over 0.5 folds. The qRT-PCR results were in accordance with the microarray outcomes. Conclusion The differentially expressed miRNA of CD4+T lymphocyte may participate in the occurring and developing of UAP.