| 不同培养代数大鼠骨髓基质细胞向神经元样细胞转分化能力的比较 |
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| 引用本文： | 陈谦,陈静,崔颜宏,庄琴,胡萍萍,龚爱华,张志坚. 不同培养代数大鼠骨髓基质细胞向神经元样细胞转分化能力的比较[J]. 神经解剖学杂志, 2009, 25(1) |
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| 作者姓名： | 陈谦 陈静 崔颜宏 庄琴 胡萍萍 龚爱华 张志坚 |
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| 作者单位： | 1. 江苏大学基础医学与医学技术学院,组织胚胎学教研室,镇江,212013
2. 江苏大学基础医学与医学技术学院,细胞生物学教研室,镇江,212013 |
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| 摘 要： | 为确定诱导大鼠骨髓基质细胞(BMSCs)向神经元样细胞转分化的适宜培养代数,本研究比较了不同培养代数的BMSCs的转分化潜能。首先体外培养大鼠BMSCs并传代,于不同的培养代数利用碱性成纤维细胞生长因子(bFGF)、神经生长因子(NGF)、全反式维甲酸(RA)、音猬因子(Shh)诱导分化,随后运用免疫荧光法和免疫印迹法检测诱导细胞中的神经元样细胞。结果显示骨髓基质细胞体外培养1至3代时能够被诱导分化为神经元样细胞,培养至第7代时丧失转分化为神经元样细胞的潜能。上述结果提示:传代次数增多将引起BMSCs自发分化,使其向神经元样细胞转分化的能力下降,因而BMSCs培养至第3代时适宜进行诱导分化。
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| 关 键 词： | 骨髓基质细胞 分化 神经元样细胞 传代 大鼠 |
Comparison of transdifferential potential of rat BMSCs at different passages to neuron-like cells in vitro |
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| Chen Qian,Chen Jing,Cui Yanhong,Zhuang Qin,Hu Pingping,Gong Aihua,Zhang Zhijian. Comparison of transdifferential potential of rat BMSCs at different passages to neuron-like cells in vitro[J]. Chinese Journal of Neuroanatomy, 2009, 25(1) |
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| Authors: | Chen Qian Chen Jing Cui Yanhong Zhuang Qin Hu Pingping Gong Aihua Zhang Zhijian |
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| Affiliation: | Chen Qian1,Chen Jing1,Cui Yanhong1,Zhuang Qin1,Hu Pingping1,Gong Aihua2,Zhang Zhijian1(1Department of Histology and Embryology,2Department of Cell Bilology,School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang 212013) |
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| Abstract: | To determine the best time for transdifferentiating rat tbone marrow stromal cells (BMSCs) into neuron-like cells,we compared the transdifferential potential of rat BMSCs at different passages. Rat BMSCs were harvested from normal rat bone marrow,then purified and passaged. The agents including bFGF,NGF,RA and Shh were used to induce the transdifferentiation of BMSCs at 3 different passages. The immunofluorescence and Western blotting were applied to detect the neuron-like cells. The results showed that rat... |
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| Keywords: | bone marrow stromal cells neuron-like cells transdifferentiation passage rat |
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