| 直肠癌循环肿瘤细胞与原发肿瘤组织基因表达谱的差异分析 |
| |
| 引用本文： | 许扬梅,龚福生,陈路川,应敏刚,郑秋红. 直肠癌循环肿瘤细胞与原发肿瘤组织基因表达谱的差异分析[J]. 中国肿瘤生物治疗杂志, 2017, 24(4): 348-354. DOI: 10.3872/j.issn.1007-385X.2017.04.003 |
| |
| 作者姓名： | 许扬梅 龚福生 陈路川 应敏刚 郑秋红 |
| |
| 作者单位： | 福建医科大学附属肿瘤医院福建省肿瘤医院国家临床重点肿瘤专科福建省肿瘤生物治疗重点实验室,福建福州,350014 |
| |
| 基金项目： | 福建省医学创新基金资助项目（No.2015-CXB-4）；福建省自然科学基金资助项目（No.2016J01436）；国家临床重点专科建设基金资助项目(No.2015-544) |
| |
| 摘 要： | 目的:通过直肠癌循环肿瘤细胞(circulating tumor cell,CTC)与原发肿瘤组织基因表达谱的差异分析,筛选直肠癌CTC的特异性基因.方法:选取2015年9月至2015年12月福建省肿瘤医院收治的直肠癌患者4例,取肿瘤组织和相应的癌旁组织并采集外周血20 ml.应用流式细胞术检测血液CTC,抽提癌组织和CTC的总RNA,采用人全转录组表达谱芯片检测CTC及相应肿瘤组织mRNA表达谱,经聚类分析筛选CTC特异性基因,并采用生物分子注释系统MAS软件对其进行基因功能及其相关的信号通路分析.结果:4例直肠癌患者检测到CTC数目分别是67、78、53和120个.筛选出肿瘤与癌旁组织差异基因共7 755个、CTC与白细胞差异基因共567个,肿瘤组织及CTC差异基因交集后共同上调或下调的特异性基因共36个,其中上调16个、下调20个(P＜0.05或P＜0.01).CTC特异性基因与肿瘤转移和干细胞功能有关,涉及与细胞转移、干细胞功能和免疫功能相关的22条信号通路.结论:CTC与原发肿瘤基因相比,具有独特的表达谱,是肿瘤复发和转移的重要基础.
|
| 关 键 词： | 直肠癌 循环肿瘤细胞 基因表达谱 |
| 收稿时间： | 2017-01-02 |
| 修稿时间： | 2017-03-25 |
Analysis of differentially expressed profiles of circulating tumor cells and primary tumor tissues in rectal cancer |
| |
| XU Yangmei,GONG Fusheng,CHEN Luchuan,YING Mingang and ZHENG Qiuhong. Analysis of differentially expressed profiles of circulating tumor cells and primary tumor tissues in rectal cancer[J]. Chinses Journal of Cancer Biotherapy, 2017, 24(4): 348-354. DOI: 10.3872/j.issn.1007-385X.2017.04.003 |
| |
| Authors: | XU Yangmei GONG Fusheng CHEN Luchuan YING Mingang ZHENG Qiuhong |
| |
| Affiliation: | Key Laboratory of Tumor Biotherapy of Fujian Province, National Clinical Key Specialty of Tumor, Tumor Hospital of Fujian Province, Tumor Hospital Affiiliated to Fujian Medical University, Fuzhou 350014, Fujian, China,Key Laboratory of Tumor Biotherapy of Fujian Province, National Clinical Key Specialty of Tumor, Tumor Hospital of Fujian Province, Tumor Hospital Affiiliated to Fujian Medical University, Fuzhou 350014, Fujian, China,Key Laboratory of Tumor Biotherapy of Fujian Province, National Clinical Key Specialty of Tumor, Tumor Hospital of Fujian Province, Tumor Hospital Affiiliated to Fujian Medical University, Fuzhou 350014, Fujian, China,Key Laboratory of Tumor Biotherapy of Fujian Province, National Clinical Key Specialty of Tumor, Tumor Hospital of Fujian Province, Tumor Hospital Affiiliated to Fujian Medical University, Fuzhou 350014, Fujian, China and Key Laboratory of Tumor Biotherapy of Fujian Province, National Clinical Key Specialty of Tumor, Tumor Hospital of Fujian Province, Tumor Hospital Affiiliated to Fujian Medical University, Fuzhou 350014, Fujian, China |
| |
| Abstract: | |
| |
| Keywords: | rectal cancer circulating tumor cells (CTCs) gene expression profile |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国肿瘤生物治疗杂志》浏览原始摘要信息 |
|
点击此处可从《中国肿瘤生物治疗杂志》下载全文 |
|
