硫磺熏蒸对人参抗应激和免疫调节作用的比较研究

目的 考察硫磺熏蒸对人参抗应激和免疫调节作用的影响。方法 抗应激作用分别通过常压耐缺氧实验和负重游泳实验，观察硫熏前后人参对小鼠耐缺氧及抗疲劳作用。免疫调节作用通过环磷酰胺（CTX）造成小鼠免疫缺陷模型，用血液分析仪检测白细胞总数（WBC）；ELISA法检测免疫细胞因子IL-2、IL-6、IL-8和IFN-γ水平；流式细胞仪检测T淋巴细胞亚群、B淋巴细胞；取胸腺、脾脏计算脏器指数。结果 同剂量组相比，硫熏组的脾指数、胸腺指数、WBC、IFN-γ、CD4*、CD19*水平显著降低（P<0.05~0.01）；此外，硫熏低剂量组的耐缺氧时间、硫熏高剂量组负重游泳时间、IL-2、IL-6水平也显著降低（P<0.05~0.01）。结论 硫磺熏蒸可使人参抗应激和免疫调节作用下降，不利于临床发挥其补气的功效。 

Comparison of Anti-stress and Immunomodulatory Activities Between Ginseng and its Sulfur-Fumigated Sample

OBJECTIVE To study the anti-stress and immunomodulatory activities of ginseng and its sulfur-fumigated sample. METHODS For anti-stress study， bearing hypoxia at normal pressure and load swimming tests were conducted， while for immunomodulatory activity study， cyclophosphamide (CTX) induced immunedeficient mouse model was used， and the index of spleen and thymus， the contents of WBC， CD3*， CD4*， CD8*， CD19* in blood and IL-2，IL-6， IL-8，IFN-γin serum were detected. RESULTS The hypoxia tolerance time， the index of spleen and thymus， and the contents of WBC， IFN-γ， CD4*and CD19*of mice administered with sulfur-fumigated ginseng at low dosage significantly decreased when compared with that administered with non-fumigated ginseng. In addition， at high dosage， the load swimming time， the index of spleen and thymus， and the contents of WBC， IL-2， IL-6， IFN-γ， CD3*， CD4*， CD8*， CD19* of mice administered with sulfur-fumigated ginseng were also decreased significantly when compared with that of non-fumigated ginseng. CONCLUSION All these results suggested that sulfur-fumigation could significantly affect the pharmacological activities of ginseng.