积雪草酸对高血脂小鼠骨量丢失的保护作用

目的探讨积雪草酸对高血脂小鼠骨量丢失的保护作用。方法 60只8周龄SPF级雄性C57BL/6 J小鼠按体重随机分为6组,每组10只:空白对照组[CON,生理盐水:5 m L/(kg·d)]、高脂饲料组[HFD,生理盐水:5 m L/(kg·d)]、高脂饲料+低剂量积雪草酸组[HFD+AA-L:5 mg/(kg·d)]、高脂饲料+中剂量积雪草酸组[HFD+AA-M:10 mg/(kg·d)]、高脂饲料+高剂量积雪草酸组[HFD+AA-H:20 mg/(kg·d)]、高脂饲料+辛伐他汀组[HFD+SIM:20 mg/(kg·d)]。除空白组之外,其他组别采用高脂饲料连续喂养16周并同时给药进行干预。实验结束后,经眼眶采血收集血清、两侧股骨和胫骨。结果高脂饲料引起小鼠血清胆固醇(TC)、丙二醛(MDA)和I型胶原C末端肽(CTX-I)水平升高,超氧化物歧化酶(SOD)和I型前胶原氨基端前肽(PINP)水平下降(均P0.05),且胫骨近端骨密度下降,骨微结构受损,骨形成率下降,其股骨生物力学指标降低。HFD+AA-M、HFD+AA-H和HFD+SIM组均可明显降低小鼠血清TC、MDA、CTX-I水平,升高SOD和PINP水平(均P0.05),增加骨密度和改善骨微结构,提高骨生物力学指标,HFD+AA-H组效果稍优于HFD+AA-M,但稍逊于HFD+SIM组。HFD+AA-L组小鼠血指标、骨密度、骨微结构和骨生物力学均无明显改善(P0.05)。结论积雪草酸[10、20 mg/(kg·d)]灌胃给药可预防高血脂小鼠的骨量丢失,其机制可能通过促进骨形成,抑制骨吸收,增加机体的抗氧化能力。

The protective effect of asiatic acid on bone loss in mice with hyperlipidemia

Objective To investigate the protective effect of asiatic acid on bone loss in mice with hyperlipidemia. Methods Sixty 8-week-old male SPF C57 BL/6J mice were randomly divided into 6 groups, with 10 mice per group: CON group (saline: 5 ml/kg/d), HFD group (saline: 5 ml/kg/d), HFD+AA-L group (AA: 5 ml/kg/d), HFD+AA-M group (AA: 10 ml/kg/d), HFD+AA-H group (AA: 20 ml/kg/d), and HFD+SIM group (SIM: 20 ml/kg/d). Mice in each group were fed with high-fat diet for 16 weeks except in CON group. Meanwhile, asiatic acid and simvastatin were orally administered for intervention. At the end of the experiment, the serum, bilateral femurs and tibias were collected. Result After feeding with high-fat diet, the serum levels of cholesterol (TC), malondialdehyde (MDA), and C-telopeptide of type I collagen (CTX-I) increased, and the serum levels of superoxide dismutase (SOD) and propeptide of type I procollagen (PINP) decreased. Bone mineral density decreased, bone microstructure was destroyed in the proximal tibias, and the value of biomechanical parameters of the femur decreased. The levels of TC, MDA, and CTX-I decreased in HFD+AA-M, HFD+AA-H, and HFD+SIM group (P<0.05), but the levels of SOD and PINP increased (P<0.05). Bone mineral density increased, bone microstructure improved, and the biomechanical indexes increased. The effect in HFD+AA-H group was slightly better than in HFD+AA-M group, but slightly less than in HFD+SIM group. The serum parameters, bone mineral density, bone microstructure, and bone biomechanical indexes were not changed in HFD+AA-L group. Conclusion Oral administration of asiatic acid (10, 20mg/kg/d) prevents bone loss via promoting bone formation, inhibiting bone absorption, and increasing the antioxidant capacity in mice with hyperlipidemia.