生地黄汤对卵巢早衰大鼠骨代谢的干预作用

目的研究生地黄汤对卵巢早衰大鼠骨代谢的影响。方法 48只成年雌性SD大鼠,随机分为空白组、模型组、阳性对照组、生地黄汤高、中、低剂量治疗组。除空白组外,其余各组每日灌胃雷公藤多甙片75 mg/kg,连续造模14 d。从第15 d起,模型组灌胃生理盐水2 mL/kg;阳性对照组灌胃补佳乐1 mg/kg;高、中、低剂量治疗组灌胃生地黄汤(12、6、3 g/kg),每日1次,连续给药21天。放射免疫法测定血清中雌激素(E2)、孕酮(P)的含量;比色法检测血清中钙、磷的含量;ELISA法检测血清中碱性磷酸酶(ALP)、骨碱性磷酸酶(BALP)、1,25二羟基维生素D_3(1,25(OH)_2D_3)含量。结果与模型组比较,生地黄汤高、中剂量治疗组血清中E2、P水平升高(P0.05);生地黄汤高剂量组血清钙、磷的含量高于模型组(P0.05);生地黄汤各剂量组血清ALP、BALP含量均较模型组降低(P0.05),1,25(OH)2D3含量高于模型组(P0.01)。结论生地黄汤可以通过类雌激素样作用升高血清雌二醇水平,改善早衰卵巢功能,同时增强成骨细胞活性,使骨形成大于骨吸收,调节骨代谢平衡,改善卵巢功能减退引起骨质疏松。

Effect of raw radix rehmanniae decoction on bone metabolism in rats with premature ovarian failure

Objective To investigate the protective effect of raw radix rehmanniae decoction (RRRD) on bone metabolism in rats with premature ovarian failure. Methods A total of 48 adult female SD rats were randomly divided into control group, model group, positive control group, and high, medium, or low dose RRRD group. Except in control group, rats in other groups received tripterygium glycosides for 14 d to set up model of premature ovarian failure. From the 15 d, rats in model group received 2 mL/kg of normal saline, rats in positive control group received 1 mg/kg of Bujiale, and rats in high, medium, or low dose RRRD group received 12, 6, or 3 g/kg of raw radix rehmanniae decoction, respectively, once a day for 21 days. The content of E2 and P was detected with radioimmunoassay. Serum calcium and phosphorus were determined with colorimetric method. Serum ALP, BALP, and 1,25(OH)2D3 were determined with ELISA. Results Compared with those in the model group, the serum levels of E2 and P in the high and middle dose treatment group of RRRD increased (P<0.05). The serum contents of Ca2+ and phosphorus in the high dose group of RRRD were higher than those in the model group (P<0.05). The contents of ALP and BALP in the RRRD groups were lower than those in the model group (P<0.05). The content of 1,25(OH)2D3 in the SDHD group was higher than that in the model group (P<0.01). Conclusion RRRD increases serum estradiol level and enhance osteoblast activity in premature ovarian function, improves bone formation over bone resorption, regulates bone metabolism balance, and relieves osteoporosis caused by ovarian dysfunction.