| Chelex-100法快速提取石蜡组织中EB病毒基因组DNA |
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| 引用本文： | 文亦磊,廖文莉,张志兴,徐华,马韵.Chelex-100法快速提取石蜡组织中EB病毒基因组DNA[J].实用医学杂志,2012(11):1790-1792. |
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| 作者姓名： | 文亦磊 廖文莉 张志兴 徐华 马韵 |
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| 作者单位： | 广西医科大学病理学教研室 |
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| 基金项目： | 广西自然科学基金项目(编号:桂科自0728116) |
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| 摘 要： | 目的:以鼻咽癌石蜡组织为材料,用Chelex-100法提取鼻咽癌组织中EB病毒基因组DNA,并以此为模板扩增出EBNA-3C片段,以建立快速提取石蜡组织中EB病毒基因组DNA的方法。方法:应用原位杂交方法检测30例鼻咽癌石蜡切片组织中EB病毒编码的小RNA(EBER)的情况,应用Chelex-100法快速提取鼻咽癌组织中EB病毒基因组DNA,并以此为模板用PCR扩增出EBNA-3C片段。结果:(1)30例鼻咽癌石蜡组织中原位杂交检测EBER全为阳性。(2)用Chelex-100法从30例鼻咽癌石蜡组织中提取了EB病毒基因组DNA并成功扩增出EBNA-3C片段。结论:Chelex-100法是快速提取鼻咽癌石蜡组织中EB病毒基因组DNA的高效方法。
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| 关 键 词： | 鼻咽肿瘤 石蜡组织 EB病毒 Chelex-100 |
Extraction of EBV DNA from paraffin tissue by Chelex-100 |
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| WEN Yi-lei,LIAO Wen-li, ZHANG Zhi-xin, XU Hua, MA Yun.Extraction of EBV DNA from paraffin tissue by Chelex-100[J].The Journal of Practical Medicine,2012(11):1790-1792. |
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| Authors: | WEN Yi-lei LIAO Wen-li ZHANG Zhi-xin XU Hua MA Yun |
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| Institution: | . Department of Pathology, Guangxi Medical University, Nanning 530021, China |
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| Abstract: | Objective To establish a quick method of EBV DNA extraction from nasopharyngeal carcinoma paraffin (NPC) tissue by Chelex-100. Methods In 30 NPC cases EBER expression was assayed by in situ hybridization(ISH), EBV DNA from NPC tissue was extracted by Chelex-100 and then PCR amplification of EBNA-3C. Results (1)30 NPC cases were positive expression of EBER. (2)EBV DNA of 30 NPC cases were extracted by Chelex-100 and PCR amplification of EBNA-3C. Conclusions Chelex-100 is an efficient method to extract EBV DNA from NPC tissue. |
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| Keywords: | Nasopharyngeal neoplasms Paraffin tissue Epstein-Barr virus(EBV) Chelex-100 |
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