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| 黄芩苷对H_2O_2诱导的SH-SY5Y细胞损伤的保护作用及其对Trx表达的影响 | |
| 引用本文： | 颜明,刘洁婷,李洪志,马恩龙,吴宜艳,郭艳芹,赵冰海,袁晓环.黄芩苷对H_2O_2诱导的SH-SY5Y细胞损伤的保护作用及其对Trx表达的影响[J].中国生化药物杂志,2012,33(5):574-577. |
| 作者姓名： | 颜明 刘洁婷 李洪志 马恩龙 吴宜艳 郭艳芹 赵冰海 袁晓环 |
| 作者单位： | 1. 沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016;牡丹江市食品药品检验检测中心,黑龙江牡丹江157011 2. 牡丹江医学院黑龙江省抗纤维化生物治疗重点实验室,黑龙江牡丹江,157011 3. 沈阳药科大学生命科学与生物制药学院,辽宁沈阳,110016 |
| 基金项目： | 黑龙江省研究生创新科研项目资金(YJSCX2011-295HLJ);黑龙江省教育厅重点项目(11551z017);黑龙江省自然科学基金(D2007-100) |
| 摘 要： | 目的探讨黄芩苷对H2O2诱导的SH-SY5Y细胞损伤的保护作用及其作用机制。方法采用体外培养细胞的方法,建立SH-SY5Y细胞H2O2损伤模型。分设药物组(50,100,200μmol/L)、H2O2损伤组、阴性对照组。采用MTT法检测细胞活力,采用实时荧光定量PCR法检测各组细胞的Trx表达的变化。结果同H2O2损伤组比较,黄芩苷能明显减轻H2O2引起的SH-SY5Y细胞的损伤,同时Trx表达升高。结论黄芩苷对H2O2诱导的SH-SY5Y细胞损伤具有明显的保护作用,其作用机制可能是黄芩苷通过上调Trx表达,起到抗氧化作用,继而起到抗凋亡的作用。 |
| 关 键 词： | 黄芩苷 SH-SY5Y细胞 H2O2 Trx |
Effect of baicalin on H2O2-induced SH-SY5Y cell injury and its influence on Trx expression |
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| YAN Ming , LIU Jie-ting , LI Hong-zhi , MA En-long , WU Yi-yan , GUO Yan-qing , ZHAO Bing-hai , YUAN Xiao-huan.Effect of baicalin on H2O2-induced SH-SY5Y cell injury and its influence on Trx expression[J].Chinese Journal of Biochemical Pharmaceutics,2012,33(5):574-577. | |
| Authors: | YAN Ming LIU Jie-ting LI Hong-zhi MA En-long WU Yi-yan GUO Yan-qing ZHAO Bing-hai YUAN Xiao-huan |
| Institution: | 1.Institute of Life Sciences and Biological Pharmacy,Shenyang Pharmaceutical University,Shenyang110016,China;2.Heilongjiang Key Laboratory of Anti-fibrosis Biotherapy, Mudanjiang Medical College,Mudanjiang 157011,China;3.Mudanjiang Institute for Food and Drug Control,Mudanjiang 157011,China) |
| Abstract: | Purpose To study the effect of baicalin on H2O2-induced SH-SY5Y cell injury and its mechanism.Methods Using SH-SY5Y cell line to establish H2O2-induced SH-SY5Y cell injury model.We design four groups(0,50,100,200 μmol/L) and test cell viability by MTT assay and Trx mRNA expression levels by Real-time quantitative PCR.Results Compared with the H2O2 injury group,baicalin can significantly reduce the H2O2-induced SH-SY5Y cell damage,while Trx,expression was increased.Conclusion Baicalin can protect SH-SY5Y cell from H2O2-injury and its mechanism of baicalin-protected may be increased Trx,and then play the anti-apoptotic role. |
| Keywords: | Baicalin SH-SY5Y cells H2O2 Trx |
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