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| 蝎毒对白血病HL—60细胞生长的抑制和诱导凋亡作用研究 | |
| 引用本文： | 贾莉,孟秀香,孙宏丹,宋振岚,孔力,魏巍.蝎毒对白血病HL—60细胞生长的抑制和诱导凋亡作用研究[J].中药药理与临床,2001,17(6):26-28. |
| 作者姓名： | 贾莉 孟秀香 孙宏丹 宋振岚 孔力 魏巍 |
| 作者单位： | 1. 大连医科大学检验系临床血液教研室,大连,116027 2. 大连医科大学检验系组胚教研室,大连,116027 3. 大连医科大学检验系临床生化教研室,大连,116027 |
| 摘 要： | 目的：研究蝎毒对HL－60细胞的生长抑制和诱导凋亡作用，并探讨其作用机制。方法：应用MTT法观察蝎毒对细胞的抑制作用，光镜、荧光显微镜和透射电镜观察细胞结构的改变，原位末端标记检测DNA断裂，流式细胞术分析凋亡细胞和bcl-2蛋白表达。结果：蝎毒可明显地抑制HL－60细胞生长和诱导细胞凋亡，作用在一定范围内呈对浓度和时间的依赖性，蝎毒并能下调HL－60细胞bcl-2蛋白表达。结论：蝎毒能抑制HL－60细胞生长和诱导细胞凋亡，其作用机制可能与bcl－2蛋白表达的下降有关。 |
| 关 键 词： | 蝎毒 细胞凋亡 HL－60细胞 bcl－2基因 白血病 |
Studies of Buthus martensii Karsch (BMK) on inhibition of cell growth and induction of apoptosis in leukemic HL-60 cell line |
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| Jia Li,Meng Xiuxiang,Sun Hongdan,et al..Studies of Buthus martensii Karsch (BMK) on inhibition of cell growth and induction of apoptosis in leukemic HL-60 cell line[J].Pharmacology and Clinics of Chinese Materia Medica,2001,17(6):26-28. | |
| Authors: | Jia Li Meng Xiuxiang Sun Hongdan |
| Abstract: | To study the mechanism of BMK on inhibition of cell growth and induction of apopsis in HL 60 cell line. Methods: The growth inhibition was analysed by M TT assay, cell apoptosis by light microscopy, fluorescence microscopy, transmiss ion electron microscopy, flow cytometry and TUNEL technique, the expression of b cl 2 protein was analysed by flow cytometry. Results: The results showed that BMK could inhib it cell growth and induce apoptosis obviously, and the effect of BMK was dose an d time dependent within a limited range. Flow cytometry showed that BMK could in hibit the expression of bcl 2 protein. Conclusion: BMK could inhibit HL 60 cell line g rowth and induce apoptosis, and the mechanism of effect occured through the expr ession of bcl 2 protein. |
| Keywords: | Buthus martensii Karsch apoptosis H L 60 bcl 2 gene |
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