响应面优化酶法提取石榴皮多糖及其抗氧化活性研究

目的 采用响应面法优化石榴皮多糖的酶法提取工艺,并对石榴皮多糖体外抗氧化活性进行研究,为药物制剂和功能性食品寻找新的生物成分。方法 采用RSM Box-Behnken设计法,考察酶解时间、液料比、加酶量对石榴皮多糖提取率的影响。用酶标仪测定DPPH自由基清除作用、羟自由基清除作用、超氧阴离子自由基清除活性和还原力测定。结果 最佳提取条件为：酶解温度为55℃,pH为5,酶解时间为88 min,液料比为22:1mL/g,加酶量为0.93%。在最佳提取条件下,石榴皮多糖得率为（22.31±0.07）%,与Box-Behnken设计模型的预测值22.35%很好地吻合。石榴皮多糖对DPPH（1,1-二苯基-2-吡啶基肼）自由基清除、羟自由基清除、超氧阴离子自由基清除和还原能力有明显的抗氧化作用。结论 建议采用优化的酶解辅助方法提取石榴皮多糖,该法制得的石榴皮多糖可作为一种良好的抗氧化剂。

Optimization of enzyme assisted extraction of polysaccharides from pomegranate peel by response surface methodology and their antioxidant potential

Objective

To optimize the enzymatic extraction conditions of polysaccharides from pomegranate peel using response surface methodology (RSM), and invest the anti-oxidant activity of pomegranate peel polysaccharide in vitro for seeking novel biological components used as pharmaceutical products and functional foods.

Methods

Effects of enzymolysis time, ratio of water to raw material, and the dosage of cellulase on the extraction yield of pomegranate peel polysaccharide were fully examined by Box-Behnken design of RSM. DPPH radical scavenging activity, hydroxyl radicals scavenging activity, superoxide anion radical scavenging activity, and reducing power assay were measured by microplate reader.

Results

The optimal extraction conditions were as follows: enzymolysis temperature 55 °C, pH 5.0, enzymolysis time 88?min, ratio of water to raw material 22:1?mL/g, and dosage of cellulose 0.93%. Under the optimal extraction conditions, the yield of pomegranate peel polysaccharide was (22.31?±?0.07)%, which was well matched with the predicted value 22.35% of the Box-Behnken design model. Also, pomegranate peel polysaccharide demonstrated appreciable anti-oxidant potential on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging and reducing power in vitro.

Conclusions

An optimized enzymolysis assisted method was proposed for the extraction of pomegranate peel polysaccharide, which can be used as a good anti-oxidant.