| 胰腺癌细胞株PANC-1中肿瘤干细胞的体外耐药性研究 |
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| 引用本文： | 安勇,姚捷,卫积书,陆子鹏,蔡辉华,戴存才,钱祝银,徐泽宽,苗毅.胰腺癌细胞株PANC-1中肿瘤干细胞的体外耐药性研究[J].外科理论与实践,2010,15(1):7-11. |
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| 作者姓名： | 安勇 姚捷 卫积书 陆子鹏 蔡辉华 戴存才 钱祝银 徐泽宽 苗毅 |
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| 作者单位： | 1. 南京医科大学第一临床医学院,普外科实验室,江苏,南京,210029
2. 南京医科大学第一临床医学院,普外科,江苏,南京,210029 |
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| 基金项目： | 江苏省2008年度普通高校研究生科研创新计划: |
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| 摘 要： | 目的:根据肿瘤干细胞学说理论,探讨胰腺癌耐药的新机制。方法:通过胰腺癌肿瘤干细胞表面标记途径和侧群(side population,SP)细胞途径,分离出人胰腺癌细胞株PANC-1中的SP/NSP(非SP)细胞及CD44+CD24+/CD44-CD24-细胞亚群,用MTT检测上述各亚群细胞在体外对化疗药物耐受的差异,用AnnexinV-PI双染法检测2种肿瘤细胞的抗凋亡能力,并采用实时荧光定量PCR检测两者耐药基因ABCG2、ABCB1和PLK-1表达的差异。结果:人胰腺癌细胞株PANC-1中的SP细胞比例为(7.64±0.96)%,CD44+CD24+细胞比例为(2.60±0.96)%。相对于NSP和CD44-CD24-细胞而言,SP和CD44+CD24+细胞具有更强的化疗耐受能力(P0.01)和抗凋亡能力(P0.01);荧光定量RT-PCR结果均提示,SP和CD44+CD24+细胞高表达耐药基因ABCG2、ABCB1和PLK1。结论:人胰腺癌细胞株PANC-1中SP及CD44+CD24+细胞具有更强的化疗耐受能力,研究胰腺癌肿瘤干细胞可为克服胰腺癌化疗敏感性差的现状提供新的实验基础和理论依据。
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| 关 键 词： | 胰腺癌 肿瘤干细胞 SP细胞 耐药性 |
Chemo-resistance of cancer stem cells in pancreatic cancer cell line PANC-1 in vitro |
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| AN Yong,YAO Jie,WEI Ji-shu,LU Zi-peng,CAI Hui-hua,DAI Cun-cai,QIAN Zhu-yin,XU Ze-kuan,MIAO Yi.Chemo-resistance of cancer stem cells in pancreatic cancer cell line PANC-1 in vitro[J].Journal of Surgery Concepts & Practice,2010,15(1):7-11. |
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| Authors: | AN Yong YAO Jie WEI Ji-shu LU Zi-peng CAI Hui-hua DAI Cun-cai QIAN Zhu-yin XU Ze-kuan MIAO Yi |
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| Institution: | AN Yonga,YAO Jiea,WEI Ji-shub,LU Zi-pengb,CAI Hui-huaa,DAI Cun-caib,QIAN Zhu-yinb,XU Ze-kuanb,MIAO Yib. a. Laboratory of General Surgery,b. Center for Pancreatic Surgery,The First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,China |
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| Abstract: | Objective To explore the possible mechanism of chemo-resistance of cancer stem cells in pancreatic cancer. Methods The side population (SP), non side population (NSP),CD44+CD24+ and CD44-CD24-sub-population of pancreatic cancer cell line PANC-1 were sorted by flow-cytometry assay . Chemo-sensitivity to gemcitabine in each group was determined by MTT assay. Apoptosis was determined by flow-cytometry. ABCG2/BCRP, ABCB1/MDR1 and PLK1 gene expression was evaluated by real-time PCR. Results Cytometry analysis sh... |
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| Keywords: | Pancreatic cancer Cancer stem cell Side population Chemoresistance |
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