| 南京地区TTV部分基因的克隆及序列测定 |
| |
| 引用本文： | 杨志国,许家璋,周育森,何长伦,隋云华,高蕾. 南京地区TTV部分基因的克隆及序列测定[J]. 江苏医药, 1999, 25(12): 902-903 |
| |
| 作者姓名： | 杨志国 许家璋 周育森 何长伦 隋云华 高蕾 |
| |
| 作者单位： | 1. 南京军区第八一医院肝病研究所,210002
2. 解放军军事医学科学院五所 |
| |
| 摘 要： | 目的：了解中国南京地区有无输血传播病毒（TTV）感染。方法：用套式聚合酶链反应（PCR）检测病因未明肝炎患者血清中TTVDNA，并对PCR扩增产物进行序列测定。结果：14例非甲-非戊型肝炎患者血清中共检出8例TTVDNA阳性者，对其中一株进行序列测定，该序列与日本TTV部分基因（CLON_22）和TTV中国株1相对应位置的核苷酸同源性很高，分别达97.65％和98.99％。结论：中国南京地区肝炎患者存在TTV感染。
|
| 关 键 词： | 输血传播病毒 聚合酶链反应 核苷酸序列 |
Cloning and Sequencing of Partial Genes of TTV in Nanjing |
| |
| Yang Zhiguo et al. Cloning and Sequencing of Partial Genes of TTV in Nanjing[J]. Jiangsu Medical Journal, 1999, 25(12): 902-903 |
| |
| Authors: | Yang Zhiguo et al |
| |
| Abstract: | ?Objective: To investigate the presence of TTV infection in Nanjing district. Methods:Serum samples from patients with hepatitis were detected for TTV DNA by PCR technique and the PCRproducts were sequenced. Results:TTV DNA was found in 8 of 14 cases suffering from non-A~E hep-atitis. The partial genes were cloned and sequenced from the serum of one patient. The partial sequenceof TTV from Nanjing showed 97.65% and 98.99% nucleotide identity with corresponding region ofTTV isolated in Japan and in other parts of China. Conclusion:There are hepatitis patients infected byTTV in Nanjing. |
| |
| Keywords: | TT virus Polymerase chain reaction DNA sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
