| 南京地区TTV部分基因的克隆及序列测定 |
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| 引用本文： | 杨志国,许家璋,周育森,何长伦,隋云华,高蕾.南京地区TTV部分基因的克隆及序列测定[J].江苏医药,1999,25(12):902-903. |
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| 作者姓名： | 杨志国 许家璋 周育森 何长伦 隋云华 高蕾 |
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| 作者单位： | 1. 南京军区第八一医院肝病研究所,210002
2. 解放军军事医学科学院五所 |
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| 摘 要： | 目的：了解中国南京地区有无输血传播病毒（TTV）感染。方法：用套式聚合酶链反应（PCR）检测病因未明肝炎患者血清中TTVDNA，并对PCR扩增产物进行序列测定。结果：14例非甲-非戊型肝炎患者血清中共检出8例TTVDNA阳性者，对其中一株进行序列测定，该序列与日本TTV部分基因（CLON_22）和TTV中国株1相对应位置的核苷酸同源性很高，分别达97.65％和98.99％。结论：中国南京地区肝炎患者存在TTV感染。
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| 关 键 词： | 输血传播病毒 聚合酶链反应 核苷酸序列 |
Cloning and Sequencing of Partial Genes of TTV in Nanjing |
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| Yang Zhiguo et al.Cloning and Sequencing of Partial Genes of TTV in Nanjing[J].Jiangsu Medical Journal,1999,25(12):902-903. |
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| Authors: | Yang Zhiguo |
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| Abstract: | ?Objective: To investigate the presence of TTV infection in Nanjing district. Methods:Serum samples from patients with hepatitis were detected for TTV DNA by PCR technique and the PCRproducts were sequenced. Results:TTV DNA was found in 8 of 14 cases suffering from non-A~E hep-atitis. The partial genes were cloned and sequenced from the serum of one patient. The partial sequenceof TTV from Nanjing showed 97.65% and 98.99% nucleotide identity with corresponding region ofTTV isolated in Japan and in other parts of China. Conclusion:There are hepatitis patients infected byTTV in Nanjing. |
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| Keywords: | TT virus Polymerase chain reaction DNA sequencing |
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