在胶原海绵膜上构建人表皮细胞和成纤维细胞复合移植物的实验研究

目的将原代培养的人表皮细胞和成纤维细胞，接种到一种新的载体-胶原海绵膜上，构建新的复合皮肤替代物。方法来源于人包皮的表皮细胞和成纤维细胞分别原代培养，成纤维细胞经消化后接种在胶原海绵膜上(1×10

An experimental study on the construction of composite graft with epithelia and fibroblasts on collagen sponge

OBJECTIVE: Primarily cultured human epithelia and fibroblasts were inoculated on collagen sponge to construct composite skin substitute. METHODS: Epithelia and fibroblast asts from human foreskin were primarily cultured. Fibroblasts (1 x 10(5)/cm(2)) were inoculated on collagen sponge after digestion and cultured for 3 days prior to inoculation of epithelial cells (2 x 10(5)/cm(2)) to construct composite skin grafting substitute, and it was then grafted onto full skin loss (FSL) wounds of nude mice. Collagen sponge membrane lacking cell inoculation was used as control. The wound healing was evaluated by measuring the residual wound area. Tissue samples were harvested and examined by means of histology, immunohistochemistry and electron microscopy. RESULTS: Epithelia and fibroblasts could be cultured in vitro on the carrier collagen sponge. After being grafted onto the wound, the epithelia and fibroblasts wound further proliferated and differentiated. The composite skin could stick to the carrier fairly well. There exhibited less epidermal scar proliferation and satisfactory contour of the neoskin. Integral basal membrane of the skin could be identified by immunohistochemical staining. The quality and speed of wound healing with the composite skin substitute were superior to that with collagen sponge only. CONCLUSION: The composite grafts constructed by epithelia and fibroblasts on collagen sponge could be a potential neotype composite skin substitute for the repair of FSL.