侧柏叶炮制方法研究

目的：研究侧柏叶炮制前后槲皮素含量的变化,建立侧柏叶合理的炮制方法。方法：通过HPLC法对侧柏叶中槲皮素的含量进行测定,色谱柱为Agilent Eclipse XPB-C18（4.6 mm×250 mm,5μm）;流动相为甲醇：0.2%磷酸溶液（55∶45）;流速1.0 mL·min^-1;检测波长360 nm;柱温30℃;并对侧柏叶的生品、烘制品及不同炮制品中槲皮素进行测定和比较。结果：槲皮素在0.03~0.52μg成良好的线性关系,回收率为97.5%~100.8%,RSD1.22%,槲皮素在烘制品中的含量较传统炮制品高。结论：所建立的HPLC法简便可靠、精密度高。烘制法具有和传统炮制法相接近的结果,提示其作为侧柏叶的炮制法存在一定的可行性。

Study of Cacumen Platycladi Leaf Processing Method

Objective：To study the variation in quercetin content before and after Cacumen Platycladi processing and to establish reasonable processing method.Methods：By HPLC method,we determined the content of quercetin in Cacumen Platycladi,chromatographic column of Agilent Eclipse XPB-C18（4.6 mm×250 mm,5μm）;the mobile phase of methanol：0.2% phosphoric acid solution（55∶45）;the flow rate was 1.0 mL·min-1;the detection wavelength was 360 nm;column temperature was 30℃;and the effect of quercetin on raw Cacumen Platycladi,baking products and its different traditional processed products were determined and compared.Results：Quercetin in a good linear relationship between 0.03~0.52 μL,the recovery rate was 97.5%~100.8%,RSD=1.22%.The content of quercetin in baked products was higher than that in traditional processed products.Conclusion：The established HPLC method is simple and reliable with high precision.The baking method has similar results with traditional processing method.It is feasible as a processing method on Cacumen Platycladi.