| 热休克自体胃癌细胞负载DC疫苗对患者术后细胞免疫功能的影响 |
| |
| 引用本文： | 许炳华,吕国强,沈晓明,陆树洪. 热休克自体胃癌细胞负载DC疫苗对患者术后细胞免疫功能的影响[J]. 肿瘤防治研究, 2010, 37(2): 169-171. DOI: 10.3971/j.issn.1000-8578.2010.02.012 |
| |
| 作者姓名： | 许炳华 吕国强 沈晓明 陆树洪 |
| |
| 作者单位： | 214041 江苏无锡市第三人民医院胃肠外科 |
| |
| 摘 要： | 目的 探讨热休克自体胃癌细胞负载的树突状细胞(DC)疫苗对胃癌术后细胞免疫功能的影响。方法 从胃癌患者外周血单个核细胞中诱导DC，并用重组人粒细胞-巨噬细胞集落刺激因子 (rhGM-CSF)和白介素4(rhIL-4)刺激活化，经负载热休克凋亡的自体胃癌细胞制备DC疫苗。将32例胃癌术后化疗患者随机分为DC疫苗治疗组16例，另16例作为对照组，仅作常规化疗。对两组病例治疗前后T细胞亚群及自然杀伤（NK）细胞进行观察比较。结果 DC疫苗治疗后外周血CD3+、CD3+CD4+、CD3+CD8+及NK细胞比率较治疗前明显升高(P<0.05)，且明显高于对照组化疗后的相应指标(P<0.05)。结论 胃癌术后化疗时行热休克凋亡自体胃癌细胞负载的DC疫苗治疗，可改善患者的细胞免疫水平。
|
| 关 键 词： | 热休克 树突状细胞 疫苗 胃癌 化疗 |
| 收稿时间： | 2008-09-03 |
| 修稿时间： | 2009-03-13 |
Effects of Dendritic Cells Pulsed with Heat-shocked Autologous Gastric Cancer Cells Lysat on Cellular Immunity of Postoperative Patients with Gastric Cancer |
| |
| XU Bing-hua,LV Guo-qiang,SHEN Xiao-ming,LU Shu-hong. Effects of Dendritic Cells Pulsed with Heat-shocked Autologous Gastric Cancer Cells Lysat on Cellular Immunity of Postoperative Patients with Gastric Cancer[J]. Cancer Research on Prevention and Treatment, 2010, 37(2): 169-171. DOI: 10.3971/j.issn.1000-8578.2010.02.012 |
| |
| Authors: | XU Bing-hua LV Guo-qiang SHEN Xiao-ming LU Shu-hong |
| |
| Affiliation: | Department of Gastrointestinal Surgery ,The Third People’s Hospital of Wuxi, Wuxi 214041, China |
| |
| Abstract: | Objective To investigate the effects of dendritic cells(DCs ) pulsed with heat-shocked autologous gastric cancer cells lysat on the cellular immunity of the postoperative patients with gastric cancer.Methods DCs isolated from peripheral blood mononuclear cell of gastric cancer patients were cultured and proliferated in vitro by using rhGM-CSF and rhll-4,and then were pulsed with heat-shocked autologous gastric cancer cells lysates.Thirty two postoperative patients with gastric cancer were randomly divided i... |
| |
| Keywords: | Heat-Shock Dendritic cells Vaccine Gastric cancer Chemotherapy |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《肿瘤防治研究》浏览原始摘要信息 |
|
点击此处可从《肿瘤防治研究》下载免费的PDF全文 |
