1,25-二羟维生素D3对骨髓破骨细胞形成和破骨细胞分化因子mRNA表达的影响

目的：观察不同浓度的1，25-(0H)2D3对骨髓单个核细胞诱导形成破骨细胞和对单个核细胞ODF mRNA表达的影响。进一步阐明骨吸收刺激因子在破骨细胞性骨吸收中的作用机制。方法：应用不同浓度的1，25-(0H)2D3(0、10^-10、10^-8、10^-6moL／L)诱导大鼠破骨细胞的形成，观察形成破骨细胞的数目，并采用原位杂交技术检测骨髓细胞ODF mRNA的表达。结果：随着l，25-(0H)：D，浓度的增加，多核细胞计数增多；ODF mRNA表达的阳性信号显著增强。结论：1，25-(0H)2D3通过调节骨髓细胞ODF mRNA的表达，影响破骨细胞的形成和功能，进而调节局部骨微环境内骨吸收与骨形成平衡的变化，影响骨组织改建。

Effects of 1,25-(OH)2D3 on stimulation of osteoclast formation and expression of ODF in murine marrow cell

AIM:To investigate the effects of 1,25-(OH) 2D 3 on stimulation of osteoclast cell formation and expression of ODF in murine marrow cell,and further clarify the mechanisms of osteoclastic bone resorption regulated by 1,25-(OH) 2D 3.METHODS:Investigate the influence of different concentrations of 1,25-(OH) 2D 3 (010 -10 ?10 -8 ?10 -6 mol/L)on the number of oteoclast formation.The effects of 1,25-(OH) 2D 3 on the expression of ODF in murine marrow cell was examined by in situ hybridzation technique.RESULTS:1,25-(OH) 2D 3 could induce recruitment of osteoclast cell.The number of oteoclast formation increased and the expression of ODF mRNA increased with increasing concentration of 1,25-(OH) 2D 3 (0?10 -10 ?10 -8 ?10 -6 mol/L).CONCLUSION:1,25-(OH) 2D 3 could modulate the recruitment of osteoclast and the expression of ODF mRNA in murine marrow cell,thus adjust the bone metabolism.