肾癌组织中微血管密度、DNA含量检测的临床意义

目的:探讨肾癌组织内微血管密度(IMD)、DNA含量的临床意义。方法:采用免疫组织化学(LSAB法)方法及图像分析技术对76例肾癌患者手术切除的标本进行IMD和DNA含量测定,并分析其与预后间的关系。结果:不同肾癌细胞类型、病理分级之间IMD值比较差异无显著性(P>0.05);随临床分期的升高,IMD增高(P<0.05)。转移组IMD值明显高于非转移组(P<0.01);随访5年内死亡者IMD、DNA含量均高于存活5年以上者(P<0.05)。Spearman相关分析研究表明:IMD与DNA蛋白含量具正相关性。结论:IMD值的变化与肾癌恶性程度相关,较高的IMD和DNA含量常提示患者预后较差。

Investigation on Intratumor Microvessel Density(IMD) and DNA Content in Renal Cell Carcinoma

Objective: To investigate the association among intratumor microvessel density(IMD), DNA content and prognosis in the patients with renal cell carcinoma. Methods: The renal cell carcinoma of 76 cases were studied by immunohistochemical staining (LSAB method) for IMD and by a computerized image analysis system for DNA content. The correlation among IMD, DNA content and renal cell carcinoma type, Robson stage, clinical grade, metastasis, prognosis was studied. Results: No correlation was detected among different cell type, clinical grade and expression of IMD( P > 0. 05). IMD levels increased with Robson stages ( P < 0. 05). IMD was significantly higher in tumors with metastasis (P < 0. 01) compared with that in tumors without metastasis. IMD and DNA contents of the 5 year survivals were significantly lower than those of the death within 5 years ( P < 0. 05). IMD has a positive correlation with DNA content in renal cell carcinoma patients after nephrectomy. Conclusion: IMD is related with tumor behavior in renal cell carcinoma patients after radical nephrectomy. IMD and DNA contents are of great prognostic significance for renal cell carcinoma patients after radical nephrectomy.