Nm23-H1和p53基因在乳腺癌细胞增殖及转移评估中的价值研究

目的探讨乳腺癌中nm23-H1、p53基因与肿瘤细胞增殖活性、淋巴结转移之间的关系。方法应用免疫组化技术检测乳腺癌组织中nm23-H1、p53基因蛋白及增殖指标k i-67的表达,结合临床及病理指标,探讨其临床意义。结果乳腺癌组织中nm23-H1、p53蛋白阳性表达率分别为50.8%(61/120)和60.0%(72/120),nm23-H1蛋白的表达与增殖程度(k i-67)和腋窝淋巴结转移有关,k i-67低增殖组nm23-H1阳性表达率为68.0%(49/72),而k i-67高增殖组nm23-H1阳性表达率则为25.0%(12/48),差异有显著性(P<0.05)。无合并淋巴结转移组中nm23-H1阳性表达率为72.2%(39/54),明显高于淋巴结转移组的阳性率33.3%(22/66)(P<0.05)。p53蛋白表达与雌激素受体有关,雌激素受体阳性组p53阳性阳性表达率为55.8%(47/86),阴性组阳性表达率为73.5%(25/34),差异有显著性(P<0.05)。结论nm23-H1和p53基因均与乳腺癌生物学行为有关,检测它们在乳腺癌中的表达可用来评估肿瘤是否具转移和侵袭能力。

Significance of nm23-H1 and p53 gene expression in predicting the proliferation and metastasis activity of breast cancer

Objective To evaluate the significance of nm23-H1 and p53 expression in predicting the proliferation and metastasis activity of breast cancer.Method The expression of nm23-H1,p53 and ki-67 in breast cancer was observed using immunohistochemical S-P technique in 120 patients.Results Nm23-H1 protein was found in 50.8%(61/120) of breast cancers,while p53 protein in 60.0%(72/120) of breast cancers.Expression of nm23-H1 correlated with ki-67 proliferation activity or regional axillary lymph node metastasis(P<0.05),and was higher in patients with ki-67 low proliferation activity(68.0%,49/72) than those with ki-67 high proliferation activity(25.0%,12/48).In addition,the expression of nm23-H1 in patients with lymph node metastasis(33.3%) was significantly lower than that those without lymph node metastasis(72.2%).There was a significant difference of p53 expression between those with ER positive(55.8%)and those with ER negative(73.5%).Conclusion Nm23-H1 and p53 expression was associated with biological behavior of breast cancer,and may be used for predicting metastasis potential.