| 乳酸菌超氧化物歧化酶基因的克隆与特性分析 |
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| 引用本文： | 王小英,马文丽,郑文岭. 乳酸菌超氧化物歧化酶基因的克隆与特性分析[J]. 海南医学院学报, 2009, 15(6): 548-552 |
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| 作者姓名： | 王小英 马文丽 郑文岭 |
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| 作者单位： | 1. 南方医科大学基因工程研究所,广东,广州,510515;海南医学院分子生物学重点实验室,海南,海口,571101
2. 南方医科大学基因工程研究所,广东,广州,510515 |
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| 基金项目： | 海南医学院重点学科项目 |
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| 摘 要： | 目的：克隆乳酸菌超氧化物歧化酶（SOD）基因并进行特性分析。方法：根据已报道的乳酸菌SOD基因序列，采用PCR技术获得SOD碱基序列，将所得的PCR产物插入克隆载体pMD-18T中，重组质粒经酶切，PCR鉴定及测序，获得的序列进行生物信息学分析。结果：成功克隆了SOD基因，序列分析表明，该SOD基因由621bp组成，编码206个氨基酸残基，蛋白分子量为23KD，等电点为4．96。结论：该蛋白氨基酸序列主要以α-螺旋为主。
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| 关 键 词： | 乳酸菌 超氧化物歧化酶 基因克隆 |
Clone and characteristic analysis of superoxide dismutase(SOD)in lactic acid bacteria |
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| WANG Xiao-ying,MA Wen-li,ZHENG Wen-ling. Clone and characteristic analysis of superoxide dismutase(SOD)in lactic acid bacteria[J]. Journal of Hainan Medical College, 2009, 15(6): 548-552 |
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| Authors: | WANG Xiao-ying MA Wen-li ZHENG Wen-ling |
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| Affiliation: | WANG Xiao-ying , MA Wen-li , ZHENG Wen-ling ( 1. Gene Project Institute in South Medical University Guangzhou 510515 ; 2. Key Laboratory of Molecular Biology, Hainan Medical College Haikou 571101, China) |
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| Abstract: | Objective:To clone and make characteristic analysis of superoxide dismutase(SOD)in lactic acid bacteria.Methods:Based on reported genetic sequence of SOD in lactic acid bacteria,obtained base sequence of SOD by PCR.Inserted product of PCR into cloning vector pMD-18T,and made enzyme digestion of recombinant plasmid.Implemented PCR identification and sequencing,and made bioinformatics analysis of sequence.Results:SOD gene was successfully cloned.Sequence analysis indicated the gene was 621 bp,encoding 206 amino acid residues,with the protein molecular weight as 23 KD and isoelectric point as 4.96.Conclusion:The main sequence of protein animo acid is alpha helix. |
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| Keywords: | Lactic acid Superoxide dismutase Gene cloning |
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