Development of a supersensitive polymerase chain reaction method for human T lymphotropic virus type II (HTLV-II) and detection of HTLV-II proviral DNA from blood donors in Japan |
| |
Authors: | Toshinari Hamakado Tadashi Matsumoto Yoshio Koyanagi Kazue Hayashi Kenji Fukada Tadashi Kouchiyama Tsutomu Yoshida Naoki Yamamoto |
| |
Institution: | (1) Ube Research Laboratory, Fujirebio Inc., Ube, Yamaguchi, Japan;(2) Fukuoka Red Cross Blood Center, Fukuoka, Japan;(3) Yamaguchi Red Cross Blood Center, Yamaguchi, Japan;(4) Department of Microbiology, Tokyo Medical and Dental University, School of Medicine, 113 Tokyo, Japan |
| |
Abstract: | A supersensitive polymerase chain reaction procedure was developed to detect human T-lymphotropic virus type II (HTLV-II) proviral genome. Six primer pairs covering the various regions of HTLV-II were compared and selected on the basis of specificity and sensitivity. Among them, one primer pair of the pol region of HTLV-II (II pol) was able to amplify and detect even 0.1 fg of the cloned plasmid HTLV-II DNA (seven copies) by regular ethidium bromide staining on polyacrylamide gel. By using this procedure, we screened 189 HTLV-I seropositive blood donors from Yamaguchi and Fukuoka Red Cross Blood Centers, Japan. There were four positive samples detectable with the HTLV-II-specific pol primer pair, as well as with the HTLV-I tax primer pair. The amplified DNAs of two specimens were cloned and sequenced. The sequences of the HTLV-I tax region from both specimens were identical to that of HTLV-I. On the other hand, those of the HTLV-II pol region were identical to that of HTLV-II, except for one base substitution in a clone from one subject. These results indicate that dual infection of HTLV-I and HTLV-II in the same persons occurs among Japanese blood donors. |
| |
Keywords: | HTLV-II PCR blood donor |
本文献已被 SpringerLink 等数据库收录! |
|