重组人促血小板生成素体内生物学活性的研究

目的　探讨重组人血小板生成素 (rhTPO)体内的生物学活性。方法　本研究以Babl/c纯系小鼠为实验对象 ,将其分成实验组及对照组 ,实验前采尾静脉血 ,测小鼠血小板正常值 ;然后连续 7d腹腔注射rhTPO ,并于注射后第 7天及第 14天采眶静脉血 0 .2ml,测定小鼠外周血小板数量 ,同时观察小鼠的死亡情况。结果　在实验第 7天 ,实验组小鼠外周血小板数明显高于同期对照组 (P <0 .0 5 ) ;而且随实验时间的延长 ,实验组血小板数有升高趋势。结论　rhTPO促进小鼠外周血小板的产生 ,增加外周血小板数量。

The study of biological activity of recombinant human thrombopoietin in vivo

Objective To investigate the biolgical activity of recombinant(rhTPO) human thrombopoietin in vivo.Methods Normal peripheral platelet counts were performed before experiment on sample obtained from the tail vein of purebred Babl/mice diveded into experiment group and control group.rhTPO was administered intraperitoneally for 7 days.On the seventh and the fourteeth day after injection platelet counts were performed on 0.2ml sample obtained from the supra-obitalis vein.Results On the seventh day and the fourteenth day,the platelet counts of mice treated by rhTPO were higher than by PBS(p<0.05).Moreover the platelet counts of mice in experimental group of rhTPO showed increasing direction following experimental days.Conclusions TPO has obvious biological activity in increasing platelet production.