低剂量辐射对脐血单个核细胞产生IL-2活性的影响

目的　探讨低剂量辐射对脐血单个核细胞产生的白细胞介素 2 (IL - 2 )生物学活性的影响。　方法　新鲜分离的脐血单个核细胞接受 6 2mGy的γ射线照射 ,在照射后不同培养时间 (2 4h ,48h ,72h) ,应用MTT(四甲基偶氮唑盐 )法 ,以CTLL依赖株为靶细胞检测培养上清IL - 2活性。　结果　低剂量照射后 ,脐血单个核细胞上清IL - 2活性随培养时间推移逐渐增幅 ,不同时间点比较均有显著性差异(P <0 .0 5 )。在不同培养时间 (2 4h ,48h ,72h) ,接受低剂量辐射的脐血单个核细胞培养上清IL - 2活性均显著高于未照射组 (P <0 .0 5 )。　结论　低剂量辐射可增强脐血T淋巴细胞释放的IL - 2的生物学活性 ,从而可能在脐血造血干细胞移植时增强移植物抗白血病 (GVL)效应

The effect of low dose irradiation on the expression of interleukin-2 in the supernatants of cord blood mononuclear cells

Objective To study on the effect of low dose irradiation on the biological activity of interleukin-2 (IL-2) in the supernatants of cord blood mononuclear cells. Methods Freshly isolated mononuclear cells from cord blood were irradiated with 62mGy gamma ray. At different culture time (24h, 48h, 72h) after irradiation, the levels of IL-2 in the supernatants were measured by a bioassay based on CTLL cells, which served as target cells. Results The biological activity of IL-2 in the supernatants of cord blood mononuclear cells enhanced increasingly after low dose irradiation. At different culturing time (24h, 48h, 72h),there were significantly higher biological activity of IL-2 in the supernatants of cord blood mononuclear cells irradiated with 62mGy gamma-ray as compared with those non-irradiated(All P <0.05).Conclusions It is suggested that enhancement of biological activity of IL-2 released from T lymphocytes in cord blood, which may contribute to the strengthening of the graft-versus- leukemia (GVL) response in cord blood stem cell transplantation, can be induced by low dose irradiation.