托吡酯促进体外培养神经干细胞增殖的实验研究

目的:研究托吡酯对体外培养神经干细胞的促增殖作用。方法:取新生小鼠大脑进行神经干细胞原代培养,托吡酯按0.4、2、10μmol.L-13个量级(或3种浓度)加入神经干细胞培养基中,同时设立溶媒对照组和阳性对照组,通过神经球生成数目及MTT法定量比较,分析不同浓度托吡酯对神经干细胞分裂增殖的影响。结果:2、10μmol.L-1托吡酯组神经球数目分别为20.67±4.04和49.68±3.79;MTT比色结果分别为0.3546±0.0705和0.5018±0.0369,明显高于溶媒对照组(7.34±2.31、0.2880±0.0093,P<0.01)。结论:托吡酯具有促进神经干细胞增殖的作用。

Topiramate Promotes Proliferation of Neural Stem Cells in Vitro

OBJECTIVE： To study the effects of topirmate on proliferation of neural stem cells in vitro. METHODS： Primary neural stem cells from newborn mice were cultured. The neural stem cells were incubated together with topiramate at 0.4, 2 or 10μmol · L^- 1. A vehicle control and positive control were set up, respectively. The quantity and viability of neural stem cells newly generated were analyzed by a convert phase microscope, the neurosphere numbers were counted, and neural stem cells viability was delermined and a MTT assay. RESULTS： The number of neurospheres of groups treated with topiramate at 2 and 10μmol · L^- 1 are 20.67± 4.04 ,49.68± 3.79, respectively, and the value of MTT assay of the two groups are 0.354 6 ± 0.070 5, 0. 501 8 ± 0. 036 9, it was higher than the vehicle control neural stem cells（7.34 ± 2.31,0. 288 0 ± 0. 009 3, P〈0.01）. CONCLUSION： Topiramate has the potential for the stimulation of proliferation of neural stem cells.