瘤内／瘤周注射巨噬细胞集落刺激因子或（和）干扰素—γ基因转染的 …

目的 观察巨噬细胞集落刺激因子（Ｍ－ＣＳＦ）和干扰素－γ（ＩＦＮ－γ）基因单独或联合转染的巨噬细胞对局部黑色素瘤的治疗效果及相关免疫机理。

Antitumor effect of M-CSF and/or IFN-gamma gene cotransfected-macrophages by intratumoral injection

OBJECTIVE: To investigate the in vivo antitumor effects of M-CSF and/or IFN-gamma gene transfected-macrophages and their related immunological mechanisms. METHODS: Survival time and antitumor effects in vivo of tumor-bearing mice treated with gene-transfected macrophages were observed. NK cell and CTL activity was detected with 4 hr 51Cr release assay, cytotoxicity of macrophages was detected with indirect MTT methods, activity of IL-2, TNF, IFN-gamma were detected by routine methods. RESULTS: 25% of the tumor-bearing mice after treatment with IFN-gamma gene-transfected, M-CSF and IFN-gamma gene co-transfected macrophages survived more than 3 month. The CTL and macrophage activity of the tumor-bearing mice was enhanced. Levels of TNF, IL-2, IFN-gamma in the supernatants of splenocytes induced in vitro were also increased. Histologic analyses of the tumor tissue after treatment with M-CSF and/or IFN-gamma gene-transfected macrophages showed a large amount of lymphocyte infiltration. CONCLUSION: IFN-gamma and M-CSF gene co-transfection into macrophages are proved to enhance their antitumor potential efficiently through direct augmentation of their tumoricidal cytotoxicity and induction of host antitumor immunity.