重组人生长激素对肾癌细胞株细胞周期动力学影响

目的　本实验试图阐明生长激素对于肾癌细胞有无促增殖和分化作用。方法　取对数生长期的人肾癌细胞株GRC -1,以氟尿嘧啶和 /或不同浓度的重组人生长激素 (rhGH)体外培养 ,2 4小时以后流式细胞仪测定细胞增殖周期和细胞凋亡等指标。结果　在空白对照组 ,G0 ～G1期的细胞数约占 5 3%,S期细胞数次之 ,G2 ～M期占不足 4%。在生长激素组 ,G0 ～G1期细胞数率降低 (P <0 .0 5 ) ,S期细胞百分率增高 (P <0 .0 1)。在单纯氟尿嘧啶组 ,G0 ～G1期细胞数增高 ,S期细胞数降低 ;同时加氟尿嘧啶和生长激素组与单纯氟尿嘧啶组相比 ,G0 ～G1期细胞数进一步增加 ,S期细胞数进一步降。结论　rhGH在体外作用于GRC -1细胞 ,能诱导细胞分化 ,促使静止细胞群进入增殖周期 ,并使处于DNA合成期的细胞增多 ,说明rhGH在体外有促进肾癌细胞生长增殖的作用 ,所以 ,我们推断肾癌细胞膜表面可能存在生长激素受体。氟尿嘧啶能使S期细胞数降低即抑制细胞的增殖 ,与氟尿嘧啶的作用机理相符。生长激素和氟尿嘧啶合用 ,S期细胞数进一步降低 ,说明生长激素能增强氟尿嘧啶抗肿瘤细胞生长增殖作用。

Effect of human growth hormone on cell cycle kinetics in GRC- 1 cells line in vitro

Objectives To explore the effect of human growth hormone on cell cycle kinetics and apoptosis of human renal carcinoma cell line (GRC-1 cells).Methods GRC-1 cells were cultured for 24 hour in 1640 with or without Fluorouraci,but with rhGH at a concentration f 50,100,200 ng/ml respectively.Cells undergoing apoptosis and differentiation were determined by flow cytometry(FCM).Results rhGH had significant effect on GRC-1 cell cycle rate,percent G 0～G 1 phases dropped (P<0.05),percent S phase increased (P<0.01).addition of Fluorouraci to the culture medium could drop all rates of S phase.Addition of rhGH at different concentration and Fluorouraci to the culture medium could cell drop rates of S phase deeply.Conclusions GRC-1 cells might have expression for the GH receptor.In Vitor rhGH might induce differentiation of cells GRC-1 and promote cell cycle kinetics and strengthen Fluorouraci suppressing on percent S phase.