MMP-9在胃癌中的表达及临床意义

目的探讨MMP-9在胃癌中的表达及其与胃癌生物学行为的关系。方法应用免疫组化技术SP法检测MMP-9基因蛋白在68例胃癌的表达情况，以20例非肿瘤性胃黏膜组织作对照。对随访14个月至13年的35例患者作了生存分析。结果68例胃癌组织MMP-9阳性表达率86．76％（59／158），对照组胃黏膜组织阳性表达率20．00％（4／20）。有淋巴结转移胃癌的MMP-9阳性表达率比无淋巴结转移者高，分别为95．65％、68．18％（P〈0．05）；侵及黏膜及黏膜下层、肌层和浆膜层胃癌的MMP-9阳性表达率分别为33．33％、91．30％、92．05％（P〈0．05）。MMP-9阳性者术后1、3、5、7、10年以上生存率分别为90．00％、56．67％、36．67％、10．00％和3．33％，MMP-9阴性患者术后1、3、5、7、10年以上生存率分别为100％、100％、80．00％、60．00％和40．00％。MMP-9阳性患者术后生存率比MMP-9阴性患者显著降低（P〈0.01）。结论MMP-9高表达促进肿瘤转移。MMP-9在胃癌的表达与肿瘤浸润深度及淋巴结转移有关；与患者年龄、性别无关。

The Study of Expression and Significance MMP-9 in Gastric Adenocarcinoma

Objective To detect the expression MMP-9 in gastric carcinoma and investigate the relationship with the clinicopathologic features and the prognosis of gastric carcinomas.Methods Sixty eight cases of paraffin embedded tissues from gastric carcinoma and 20 cases non-tumor gastric tissue were detected for the expression of MMP-9 by means of immunohistochemical assay.Thirty five cases were followed-up from 14 months to 13 years,in which the prognosis was analyzed.Results Of the 68 cases gastric carcinoma,59(86.76%) were positive for MMP-9.Of the 20 cases non-tumor gastric tissue,4(20.00%) were positive for MMP-9.MMP-9 positivity rate in the cases with lymph node metastasis was higher than that without lymph node metastasis and they were 95.65% and(68.18%),rspectively(P<0.05);MMP-9 was expressed in mucosa,muscularis and serosa in 33.33%,(91.30%),92.05%,(P<(0.05)).The expression of MMP-9 were not related to age and sex(P>(0.05)).The patients with MMP-9 high positivily had low survival(P<0.01).Conclusion High expression of MMP-9 promotes tumor metastasis.The expression of MMP-9 is related to invasion and lymph node metastasis of the tumor.and were not related to age and sex.