Interphase Analysis of Chromosome 11 in Human Pituitary Somatotroph Adenomas by Direct Fluorescence In Situ Hybridization |
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Authors: | Kontogeorgos George Kapranos Nikiforos |
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Institution: | (1) Department of Pathology, Hygeia Hospital, Athens, Greece;(2) Department of Pathology, General Hospital of Athens, 154 Messogion Avenue, 115 27 Athens, Greece |
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Abstract: | Chromosome 11 numerical abnormalities were detected by fluorescencein situ hybridization (FISH) technique in four surgically removed pituitary somatotroph adenomas from patients clinically associated
with acromegaly. The tumors were diagnosed by histology, immunocytochemistry, and electron microscopy, and included two densely
granulated somatotroph (DG-SM) and two sparsely granulated somatotroph (SG-SM) adenomas. For demonstration of chromosome 11,
the direct FISH technique was applied on imprints from fresh adenoma tissue fixed in acetone using an α-satellite specific
centromeric probe. The slides were studied with a fluorescence microscope and the percentages of positive nuclei with aberrant
fluorescent signals were counted. All tumors exhibited numerical chromosomal abnormalities in 8–23% of their cell population
and included nuclei containing 1–3 extra chromosome 11 copies. The SG-SM adenomas exhibited more prominent abnormalities compared
with the DG-SM adenomas. We conclude that numerical chromosome 11 abnormalities represent a frequent event among somatotroph
adenomas with a tendency for higher frequency in SG-SM adenomas. |
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Keywords: | Chromosome aberrations cytogenetics FISH technique immunocytochemistry interphase analysis pituitary ultrastructure |
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