| UV-C诱导的凋亡SMC形态学变化和形态计量学研究 |
| |
| 引用本文： | 李晓丹,李伟峰. UV-C诱导的凋亡SMC形态学变化和形态计量学研究[J]. 第一军医大学分校学报, 2002, 25(2): 81-83 |
| |
| 作者姓名： | 李晓丹 李伟峰 |
| |
| 作者单位： | 1. 第一军医大学分校内科学教研室,广东省,广州市,510315
2. 广州军区广州总医院呼吸内科,广东省,广州市,510010 |
| |
| 摘 要： | 目的 观察经UV -C照射诱导的体外凋亡动脉中膜平滑肌细胞 (SMC)形态学变化 ,并探讨二维平面上SMC细胞面积 (CA)、核面积 (NA)及核 /胞面积比 (RA)的变化。方法 应用HE染色技术在光镜下观察凋亡SMC形态变化 ;应用图象分析技术进行形态计量学研究。结果 UV -C照射可诱导SMC发生典型凋亡形态学改变 ,凋亡细胞CA、NA、RA均显著减少 (P <0 0 1) ,不同时间段亦有明显差异。结论 在UV -C诱导体外SMC凋亡的过程中 ,细胞的皱缩可能参与了凋亡的触发机制和 /或推动了凋亡的进程
|
| 关 键 词： | UV-C诱导 SMC形态学 形态计量学 研究 血管平滑肌细胞 细胞凋亡 动脉粥样硬化 |
| 文章编号： | 1009-1793(2002)02-0081-03 |
| 修稿时间： | 2002-09-06 |
Morphological changes and image analysis of apoptotic vascular smooth muscle cells induced by Ultraviolet-C in vitro |
| |
| LI Xiaodan,LI Weifeng.. Morphological changes and image analysis of apoptotic vascular smooth muscle cells induced by Ultraviolet-C in vitro[J]. Journal of Branch Campus of the First Military Medical University, 2002, 25(2): 81-83 |
| |
| Authors: | LI Xiaodan LI Weifeng. |
| |
| Abstract: | Objective To observe the morphological changes of the apoptotic artery smooth muscle cells(SMCs)induced by Ultraviolet C (UV-C)and study the changes of cellular area(CA),nuclear area(NA)and the ratio of area(RA)in two-dimensional plane. Methods HE decoration method and image analysis were used to observe the morphological changes of apoptotic SMCs. Results Cultured SMCs were found to undergo apoptosis,which was characterized by morphological changes after exposure to UV-C.The CA,NA,RA were all distinctly lower than those in normal SMCs and had a significant difference with the elapse of time. Conclusion Cell shrinkage of apoptotic SMCs perhaps participates in the trigger mechanism of the apoptosis induced by UV-C and/or promote the process. |
| |
| Keywords: | smooth muscle cells apoptosis ultraviolet cell shrinkage trigger mechanism |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
