| 雷帕霉素对HL-60细胞增殖和凋亡的影响 |
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| 引用本文： | 崔亚兰,曹诗运.雷帕霉素对HL-60细胞增殖和凋亡的影响[J].南华大学学报(医学版),2008,36(3):316-319. |
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| 作者姓名： | 崔亚兰 曹诗运 |
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| 作者单位： | 南华大学,生理教研室,湖南,衡阳,421001 |
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| 摘 要： | 目的研究雷帕霉素作用于HL-60细胞后，对细胞增殖、细胞周期及细胞凋亡率的影响。方法通过细胞生长曲线、MTT检测细胞增殖情况；免疫荧光染色、流式细胞仪检测细胞周期和凋亡率。结果雷帕霉素组与空白对照组细胞生长抑制率比较，差异有显著性（P〈0．05）；细胞培养72h后，雷帕霉素组与空白对照组细胞凋亡率比较，差异有显著性（P〈0．05）。结论雷帕霉素能抑制HL-60细胞增殖及促进其凋亡。
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| 关 键 词： | 白血病 雷帕霉素 增殖 凋亡 |
Effect of Rapamycin on the Proliferation and Apoptosis of Human Leukemia Cell Line HL-60 |
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| CUI Ya-lan,CAO Shi-yun.Effect of Rapamycin on the Proliferation and Apoptosis of Human Leukemia Cell Line HL-60[J].Journal of Nanhua University(Medical Edition),2008,36(3):316-319. |
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| Authors: | CUI Ya-lan CAO Shi-yun |
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| Institution: | Institute of Physiology;Nanhua University;Hengyang;Hunan 421001;China |
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| Abstract: | Objective To investigate the effct of Rapamycin on the proliferation,cell cycle and apoptosis of human leukemia cell line HL-60.Methods HL-60 cells were treated with various doses of rapamycin,the proliferation of HL-60 cells was detected by growth curve and MTT method.Immunofluorescent staining and flow cytometer was used for cell cycle and appoptosis detection. Results Compaired with rapamycin and control group,there is a significant difference of cell growth inhibition rate between control group and rapa... |
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| Keywords: | leukemia Rapamycin proliferation apoptosis |
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