| 用脂质体法转染COS—7细胞条件的优化 |
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| 引用本文： | 胡志远 邢桂春. 用脂质体法转染COS—7细胞条件的优化[J]. 军事医学科学院院刊, 1997, 21(2): 125-127 |
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| 作者姓名： | 胡志远 邢桂春 |
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| 作者单位： | 军事医学科学院放射医学研究所 |
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| 摘 要： | 现有的多种真核细胞基因转染方法存在繁琐和效率低的问题，我们利用脂质体包裹的方法，以瞬时表达系统中最常用的ＣＯＳ－７细胞为靶，观察了影响转梁效率的多种因素，优化了转染条件。
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| 关 键 词： | 瞬时表达 COS－7细胞 转染效率 脂质体 |
Optimization on the condition of liposome mediated transfection to COS 7 cell line |
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| Hu Zhiyuan,Xing Guichun,He Fuchu Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing. Optimization on the condition of liposome mediated transfection to COS 7 cell line[J]. Bulletin of the Academy of Military Medical Sciences, 1997, 21(2): 125-127 |
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| Authors: | Hu Zhiyuan Xing Guichun He Fuchu Institute of Radiation Medicine Academy of Military Medical Sciences Beijing |
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| Affiliation: | Hu Zhiyuan,Xing Guichun,He Fuchu Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850 |
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| Abstract: | The present transfection methods for eukaryotic cells have the problems of troublesomeness and low efficiency. Liposome mediated transfection method was used for COS 7 cell line. Factors which affected transfection efficiency were observed and the transfection condition was optimized. |
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| Keywords: | transient expression COS 7 cell line transfection efficiency liposomes |
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