Adenylate cyclase activity in human pancreatic adenocarcinoma cell lines |
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Authors: | Layla Al-Nakkash Nicholas L Simmons Jennifer M Lingard and Barry E Argent |
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Institution: | (1) Department of Physiological Sciences, University Medical School, Framlington Place, Newcastle upon Tyne, UK;(2) Pediatric Pulmonary Division, BRB, School of Medicine, C.W.R.U., 10900 Euclid Avenue, 44106-4948 Cleveland, OH;(3) Department of Biological Sciences, Faculty of Health Sciences, University of Sydney, Lidcombe, Australia |
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Abstract: | Summary
Conclusion
BxPC-3, Hs 766T, Capan-2, Panc-1, and Capan-1 cells possess receptors for VIP and β-adrenergic agonists that are functionally
coupled to adenylate cyclase. In this respect, they resemble pancreatic duct cells. However, we speculate that the process
of neoplastic transformation has either downregulated the expression of secretin receptors or led to a defect in the receptor
itself, placing a question mark over the usefulness of these adenocarcinoma cell lines as models of the pancreatic ductal
epithelium.
Background Because of the importance of ducts in pancreatic disease, we wished to establish which duct cells receptors are functional
on adenocarcinoma cell lines.
Methods We investigated the expression of agonist-stimulated adenylate cyclase activity in six human pancreatic adenocarcinoma cell
lines. Known stimulants of pancreatic ductal secretion, VIP, PHI, secretin, β-adrenergic, and dopamine, were tested.
Results For responsive cell lines, VIP was the most effective stimulant followed by adrenaline, isoprenaline, PHI, and secretin. Dopamine
was without effect. Since high concentrations of PHI and secretin were required to stimulate cyclase activity, their effect
is probably mediated by VIP receptors. Based on the degree of stimulation observed with the individual agonists, Hs 766T and
BxPC-3 were the most responsive cell lines, followed by Capan-2 and Capan-1, and finally Panc-1. MIAPaCa-2 cells did not respond
to any of the agonists tested. |
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Keywords: | Human pancreatic adenocarcinoma cell line adenylate cyclase peptide receptors adrenergic receptors |
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