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| 毛细管电泳测定微量氨基酸柱上样品堆积法研究 | |
| 引用本文： | 沈佐君,孙曾培,杨树德.毛细管电泳测定微量氨基酸柱上样品堆积法研究[J].中国生化药物杂志,2001,22(2):71-73. |
| 作者姓名： | 沈佐君 孙曾培 杨树德 |
| 作者单位： | 1. 安徽省立医院省检验中心, 2. 中国药品生物制品检定所, 3. 卫生部临床检验中心, |
| 基金项目： | 安徽省自然科学基金(项目编号00044517) |
| 摘 要： | 目的建立一种柱上样品堆积法测定微量氨基酸的方法。方法分别用运行缓冲液或用水和乙腈重组经萃取吹干的样品 ,压力法进样 5、2 5和 5 0s,高效毛细管电泳法定量分析 ,观察用不同溶剂重组对柱上样品堆积效果的影响。结果该法提高微量氨基酸检测灵敏度 10 0倍并保持柱效不变。结论用水和乙腈 (1∶1)作溶剂重组残留样品的方法获得最佳的柱上堆积效果。 |
| 关 键 词： | 毛细管电泳 氨基酸 柱上样品堆积 |
| 文章编号： | 1005-1678(2001)02-0071-03 |
| 修稿时间： | 2000年5月15日 |
A method of on-column sample stacking in capillary electrophoretic analysis of micro-scale amino acids |
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| SHEN Zuo-jun,SUN Zeng-pei,YANG Shu-De.A method of on-column sample stacking in capillary electrophoretic analysis of micro-scale amino acids[J].Chinese Journal of Biochemical Pharmaceutics,2001,22(2):71-73. | |
| Authors: | SHEN Zuo-jun SUN Zeng-pei YANG Shu-De |
| Institution: | SHEN Zuo jun 1,SUN Zeng pei 2,YANG Shu de 3 |
| Abstract: | Purpose The aim is to develop a method for the analysis of micro-scale amino acids in biological samples.Methods After having been extracted and dried,the residues were dissolved in a running buffer or a distilled water and acetonitrile.The samples were loaded into column for 5,25 or 50s at a hydrodynamic injection and were separated by capillary electrophoresis.The effect of different dissolvent on the sample stacking was observed.Results A 100-fold improvement in the amount of material that can be injected into a capillary column without loss of resolution was shown.Conclusion The marked effect of sample stacking was achieved by the using distilled water and acetonitrile(1∶1,v/v) as dissolvent. |
| Keywords: | Capillary electrophoresis Amino acids Sample stacking |
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