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脱氢表雄酮对大鼠实验性自身免疫性神经炎的影响
引用本文:谭晓冬,段瑞生,时昌文,孙若鹏. 脱氢表雄酮对大鼠实验性自身免疫性神经炎的影响[J]. 山东大学学报(医学版), 2008, 46(9): 837-841
作者姓名:谭晓冬  段瑞生  时昌文  孙若鹏
作者单位:山东省千佛山医院儿科,济南,250014;山东大学齐鲁医院儿科,济南,250812
摘    要:目的探讨脱氢表雄酮(DHEA)对大鼠实验性自身免疫性神经炎(EAN)发病的影响及其机制。方法随机将36只Lewis大鼠分为DHEA 0.5mg治疗组、2mg治疗组和对照组。治疗组于注射牛周围神经髓磷脂(BPM)抗原乳剂后第5天开始每日皮下注射DHEA,对照组皮下注射相同体积的DHEA溶媒。观察各组发病时间及临床评分,检查疾病高峰期坐骨神经组织病理及干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)阳性反应细胞,检测引流淋巴结和脾脏单个核细胞增殖反应,检测培养上清TNF-α、IFN-γ、白介素-10(IL-10)含量。 结果两种剂量DHEA治疗组均能延迟EAN发病时间(P均<0.05),减少坐骨神经炎性细胞浸润数目(P均<0.05)和IFN-γ、TNF-α阳性反应细胞数目(P均<0.05),抑制BPM刺激T淋巴细胞增殖(P均<0.05),降低培养上清中IFN-γ、TNF-α水平(P均<0.05)。2?mg治疗组疾病高峰期临床评分明显降低于对照组(P<0.05)。各组间 IL-10水平未显示明显差异(P>0.05)。结论DHEA可能通过抑制自身反应性T淋巴细胞增殖和促炎性细胞因子过度表达减轻EAN病情,2mg治疗组具有更明显的免疫治疗效果。

关 键 词:神经炎  实验性变应性  脱氢(表)雄甾酮  细胞因子类  大鼠  近交Lew
收稿时间:2008-04-17

Effects of dehydroepiandrosterone on experimental autoimmune neuritis in Lewis rats
TAN Xiao-dong,DUAN Rui-sheng,SHI Chang-wen,SUN Ruo-peng. Effects of dehydroepiandrosterone on experimental autoimmune neuritis in Lewis rats[J]. Journal of Shandong University:Health Sciences, 2008, 46(9): 837-841
Authors:TAN Xiao-dong  DUAN Rui-sheng  SHI Chang-wen  SUN Ruo-peng
Affiliation:1. Department of Pediatrics, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;2. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, China
Abstract:To explore the effects of dehydroepiandrosterone (DHEA) on the development of experimental autoimmune neuritis (EAN) and to explore its mechanism. Methods Thirty-six Lewis rats were randomly divided into the DHEA 0.5mg treatment group, the DHEA 2mg treatment group and the control group (n=12). The treatment groups were subcutaneously injected every day with DHEA and the control group was subcutaneously injected with the same volume of DHEA dissolvent from day 5 post-immunization (p.i.) to day 28 p.i. with bovine peripheral myelin(BPM)in Freund′s complete adjuvant (CFA). The effects were assessed in terms of appearance of clinical signs, clinical score, histopathology and IFN-γ and TNF-α positive cells in sciatic nerve sections, and T-cell proliferation and inflammatory cytokines (IFN-γ,TNF-α and IL-10) synthesis by draining lymph nodes and spleen cells. ResultsRats receiving DHEA in the two different therapeutic regimens displayed a significant delay in onset (P<0.05 and P<0.05), a decreased inflammatory cell infiltration into the PNS (P<0.05,P<0.05) and decreased numbers of IFN-γ and TNF-α expression cells in the PNS(P<0.05 and P<0.05 ), and reduced BPM stimulated T cell proliferation(P<0.05 and P<0.05) and IFN-γ and TNF-α secretion in the draining lymph node and spleen (P<0.05, P<0.05) compared to the control group. Only the DHEA 2mg treatment group had the significant differences of supernatant IL-10 were found among the different treatment and control groups(P>0.05).ConclusionAdministration of exogenous DHEA ameliorates the severity of EAN, presumably by suppressing the proliferation of auto-reactive T-cells and moderating the over-expression of pro-inflammatory cytokines. DHEA 2mg therapy is more effective than DHEA 0.5mg therapy.
Keywords:Neuritis  experimental allergic  Dehydroepiandrosterone  Cytokines  Rats  inbred Lew
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