梅毒血清学检测分析

目的以梅毒螺旋体明胶凝集试验（TPPA）为标准，比较临床常用的检测梅毒的两种血清学方法的优劣性。方法用非特异性的甲苯胺红不加热血清试验（TRUST）、酶联免疫吸附试验（ELISA）方法对883名性病门诊患者进行梅毒筛检，阳性标本用梅毒确证试验（TPPA）法进行确证。结果TRUST法灵敏度为71．01％（245／345），假阳性率8．92％（24／269），漏检率28．99％（100／345）；ELISA法灵敏度为99．42％（343／345），假阳性率0．58％（2／345），漏捡率为0．58（2／345）。与TRUST相比，ELISA法检测敏感度（X^2=110．49，P〈0．01）、假阳性率（X^2=25．94，P〈0．01）以及漏检率均有明显差异（X^2=110．49，P〈0．01）。结论ELISA法具有较高的灵敏度和特异性，漏检率较低，适合用于性病门诊梅毒筛查。

Serological detections of syphilis

Aim To compare two kinds of serological approaches detecting Treponema pallidum antibody baesd on TPPA methods. Methods TRUST and ELISA were used to detect 883 serum specimens from STD outpationts.TPPA method was used to detect serum specimens with either TRUST positive or ELISA positive results. Results The sensitivity,the false positive rate and the missed detection rate of TRUST were 71.01% （245/345）,8.92 %（24/269）,28.99%（100/345）,while the results of ELISA were 99.42% （343/345）, 0.58% （2/345）and 0.58 （2/345） respectively. Compared with the results of TRUST, the results of the 3 index of ELISA showed statistically significant differences （P〈0.01）. Comclusion ELISA is better than TRUST and is suitable for detection of antibody to Treponema pallidum in STD outpatients.