LC-MS/MS法分析海洛因吸食者毛发中的阿片类化合物

目的评价海洛因吸食者毛发样本的洗涤过程,并对酶解提取方法和匀碎提取方法进行比较。方法毛发样品共洗涤3.15小时后选择一种温和的酶解方法(pH6.6)和一种缓冲液提取方法(48℃、18小时),使用混合型吸附柱(MCX Oasis)进行固相萃取,通过液相色谱串联质谱(LC-MS/MS)分析方法,检测海洛因、6-MAM、乙酰可待因、可待因和吗啡等化合物的含量。结果在酶解的毛发样本中,海洛因在总吗啡中的含量是21.82%,吗啡/6-MAM的值为0.9875。在匀碎提取方法中,吗啡/6-MAM的值为0.3948。结论洗涤过程不能有效地将从外界渗透到毛发中的污染物去除。

Analysis on opioid compounds in the hair of heroin addicts with LC-MS/MS

Objective To evaluate the effects of washing process on hair samples from heroin addicts,and compare the extracting method of enzymatic digestion with homogenization of hair samples.Methods After washing samples for 3.15h,a mild enzymatic digestion procedure at pH 6.6 and a buffer incubating procedure in water bath at 48℃ during 18h was used.The samples were pre-treated by solid phase extraction using mixed mode sorbent columns(MCX Oasis).Quantitative analysis of morphine,codeine,6-acetylmorphine,heroin,acetylcodeine by chromatography-tandem mass spectrometry method was developed.Results Heroin accounted for 21.82% of the total equivalents of morphine.The ratio of morphine to 6-MAM in enzymatic digestion hair samples was 0.9875 and that in homogenized hair samples was 0.3948.Conclusion The results suggested that the procedures were not sufficient to remove the contaminants penetrating into hair from external sources.