丹参中水溶性酚酸类成分的薄层扫描测定法

应用高效薄层扫描法对丹参根中的原儿茶醛、咖啡酸、迷迭香酸及其甲酯、丹酚酸A,B和C7种有效成分进行了含量测定。选择了最佳提取分离方法,即生药用热水提取并酸化后用乙酸乙酯萃取,萃取液浓缩后进行薄层色谱分离。展开剂A:氯仿乙酸乙酯——苯—甲酸(2.4:2:1:0.6)分离原儿茶醛,展开剂B:氯仿-乙酸乙酯-苯-甲酸甲醇(1.5:2:1;1:0.1)分离其余6种成分。并用所建立的分析方法分析比较了4种丹参根中7种有效成分的含量。

SEPARATION AND QUANTITATIVE DETERMINATION OF SEVEN AQUEOUS DEPSIDES IN SALVIA MILTIORRHIZA BY HPTLC SCANNING

A new analytical method for the separation and determination of seven aqueous depsides in salria milliorrhiza by HPTLC has been developed. The seven depsides are protocatechualdehyde, caffeic acid, methyl rosmarinate, rosmarinic acid, salvianolic acid A, B and C. Using chloroform—ethyl acetate—benzene—formic acid (2.4:2:1:0.6) as developing solvent A. protocate-chualdehyde was separated; using chloroform—ethyl acetate—benzene—formic acid—methanol (1.5:2:1:1:0.1) as developing solvent B, the other six constituents were well separated. The aqueous depsides were detected at the wave lengths of λ_s=300 nm and λ_R=240. This method is simple. rapid, sensitive and accurate. The contents of seven depsides in several Salria species were determined.