刺五加叶皂苷对实验性血管性痴呆大鼠学习、记忆及病理损伤的保护作用

目的:观察刺五加叶皂苷(ASS)对大脑中动脉闭塞(MCAO)引起的大鼠实验性血管性痴呆的影响,为ASS的进一步开发提供依据.方法:Wistar大鼠随机分为假手术组、血管性痴呆模型组及ASS 低、中、高剂量组.采用MCAO法制备大鼠血管性痴呆模型,造模10 d 后ASS低、中、高剂量组大鼠腹腔注射ASS(25、50和10...

Protective effect of ASS on learning,memory and pathological injury in rats with experimental vascular dementia

Objective To observe the effect Acanthopanax senticosus saponins(ASS) on experimental vascular dementia in rats induced by middle cerebral artery occlusion(MCAO) and provide basis for further development of ASS.Methods Wistar rats were randomly divided into sham group,model group and ASS(low dose,middle dose and high dose) groups.The experimental vascular dementia model induced by MCAO was set up in rats,10 d later,they were treated by continuously intraperitoneal injection of 25,50 and 100 mg·kg-1 ASS for 20 d.The abilities of learning and memory were evaluated by Morris water maze test.After detection the ethology,the brain tissues were collected to carry out pathological analysis by light microcope and homogenate to detect the activities of acetylcholine esterase(AchE) and choline acetyl transferase(ChAT).Results In Morris water maze test,compared with model group,the escape latency and swimming distance were decreased in the 4th and 5th days(P<0.05 or P<0.01);and the ratio of the swim time in the virgin platform quadrant(tP) and the total swim time(tT) and the ratio of the swim distance in the virgin platform quadrant(dP) and the total swim distance(dT) were increased significantly(P<0.05) in 50 and 100 mg·kg-1 ASS groups.and the activity of AchE was decreased significantly(P<0.05) in 50 and 100 mg·kg-1 ASS groups;the activity of ChAT in 100 mg·kg-1 ASS group was increased significantly(P<0.05).The histopathological results indicated that there were marked focus of infarction in the cerebr-blood-supply region by middle cerebral artery,the nerve cells of the hippocampal CA1 fields of rats arranged disorderedly,the quantity of nerve cells was decreased markedly in model group.50 and 100 mg·kg-1 ASS diminished the size of focus of infarction,protected the nerve cells of the hippocampal CA1 fields from disorder,maintained the quantity of nerve cells,and improved the damage of brain.Conclusion ASS can improve the abilities of learning and memory of vascular dementia rats and protect the brain tissue from pathological changes.Its mechanism may be related to increasing the activity of ChAT,decreasing the activity of AchE,and promoting the synthesis of AchE.