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| 四逆汤抗失血性休克大鼠肠黏膜损伤 及菌群移位药效研究 | |
| 引用本文： | 陈兰英,吴刚,陈卓,董鑫鑫,段萌,王昌芹,陈金龙,刘红宁. 四逆汤抗失血性休克大鼠肠黏膜损伤 及菌群移位药效研究[J]. 中国实验方剂学杂志, 2011, 17(23): 121-124. DOI: 11-3495/R.20111011.1055.002 |
| 作者姓名： | 陈兰英 吴刚 陈卓 董鑫鑫 段萌 王昌芹 陈金龙 刘红宁 |
| 作者单位： | 1. 江西中医学院,南昌330004;中药固体制剂制造技术国家工程研究中心,南昌330006 2. 江西中医学院,南昌,330004 3. 中药固体制剂制造技术国家工程研究中心,南昌,330006 |
| 基金项目： | 国家科技重大专项项目(2009ZX09310-005) |
| 摘 要： | 目的:研究四逆汤对失血性休克大鼠肠黏膜损伤及肠道菌移位的影响。方法:动物分为空白组、失血性休克组、四逆汤高、低剂量组(按生药量计4.8,2.4 g.kg-1)。通过定血量放血建立大鼠失血性休克模型,观察复苏3 h后肠黏膜损伤程度,并取肝、脾、肾和肠系膜淋巴结进行细菌培养,测定肝门静脉血浆内毒素、动脉血浆D-乳酸(D-Lac)含量。并通过血浆肿瘤坏死因子-α(TNF-α)、总一氧化氮合酶(t-NOS)的测定进行作用机制的探讨。结果:四逆汤与模型组比较,肠黏膜病理学改变得到显著改善,细菌培养计数、内毒素及D-Lac含量显著减少。血浆t-NOS显著减少,TNF-α有降低趋势,四逆汤药效作用呈剂量依赖性增强。结论:四逆汤能够抑制肠道菌移位,减轻肠黏膜损伤。作用机制可能与抑制t-NOS,TNF-α有关。 |
| 关 键 词： | 四逆汤 失血性休克 肠黏膜损伤 肠道菌移位 |
| 收稿时间： | 2011-03-30 |
The Effect of Sini Decoction on Intestinal Mucosa Injury and Translocation of Bacteria Induced by Hemorrhagic Shock |
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| CHEN Lan-ying,WU Gang,CHEN Zhuo,DONG Xin-xin,DUAN Meng,WANG Chang-qin,CHEN Jin-long and LIU Hong-ning. The Effect of Sini Decoction on Intestinal Mucosa Injury and Translocation of Bacteria Induced by Hemorrhagic Shock[J]. China Journal of Experimental Traditional Medical Formulae, 2011, 17(23): 121-124. DOI: 11-3495/R.20111011.1055.002 | |
| Authors: | CHEN Lan-ying WU Gang CHEN Zhuo DONG Xin-xin DUAN Meng WANG Chang-qin CHEN Jin-long LIU Hong-ning |
| Affiliation: | Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China; National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China |
| Abstract: | Objective:To investigate the effect of Sini decoction(SND) on the intestinal mucosal injury and intestinal bacterial translocation induced by hemorrhagic shock.Method: The animals were randomly divided into normal group,shock model group,SND high dose group,SND low dose group.The shock model was induced by hemorrhaging for one hour and then resuscitating with shedding blood and same-volume Ringer lactate solution.The morphological changes in ileum mucosa were observed under light microscope.The intestinal b... |
| Keywords: | Sini decoction intestinal mucosal injury hemorrhagic shock translocation of intestinal bacteria |
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