荷叶铁线蕨水提液对人胚肝细胞增殖和功能的影响

目的:研究荷叶铁线蕨对人胚肝细胞增殖和功能的影响。方法:人胚肝细胞L-O2 96孔板104/孔培养,经质量浓度为1 g.mL-1荷叶铁线蕨水提液梯度稀释后处理24 h,采用MTT法检测细胞增殖情况;溴甲酚绿比色法检测白蛋白分泌水平;酶标仪法测定乳酸脱氢酶(LDH)活力。结果:荷叶铁线蕨水提液可以促进人胚肝细胞增殖,且具有剂量依赖性;在药物质量浓度为100 g.L-1时细胞白蛋白分泌量达到最高2.14 g.L-1,并显著降低了LDH的活力(P<0.05)。结论:荷叶铁线蕨水提液可以促进人胚肝细胞增殖,增强细胞保肝降酶作用。

The Proliferation and Function in Human Embryonic Hepatocytes Treated by Adiantum reniforme Extracts

Objective: To study the proliferation and function in human embryonic hepatocytes treated by Adiantum reniforme L. var. sinense Y. X. Lin extracts. Method: Human embryonic hepatocytes L-O2 10⁴in culture were treated for 24 h with 1 g·mL^-1 the aqueous extract solution. The cell proliferation was measured by the MTT assay. The albumin secretion was detected by the bromcresol green colorimetry. The activity of lactate dehydrogenase (LDH) in culturalmedia were measured by enzyme linked immunosorbent assay. Result: The extracts promoted the cell proliferation with dose-dependent. At drug concentration in 100 g·L^-1, the aqueous extract solution increase the secretion of albumin to 2. 14 g·L^-1, and decreased LDH activity. Conclusion: The extract of A. reniforme can promote the hepatic proliferation and function.