麻杏石甘汤对枸橼酸致豚鼠咳嗽的影响

目的:探讨麻杏石甘汤防治枸橼酸所致豚鼠咳嗽的作用及初步机制。方法:豚鼠随机分成正常组、模型组、可待因组、麻杏石甘汤低、中、高剂量组(2.3,6.5,14.6 g.kg-1),分别连续ig给药7 d,最后1次给药后1 h,采用枸椽酸致豚鼠咳嗽模型,以生物信息采集系统记录咳嗽信号、录音和人工计数等方法统计咳嗽次数和咳嗽潜伏期,对支气管进行病理切片观察,并检测血清过氧化氢酶活力和过氧化氢的含量。结果:模型组咳嗽次数为(53.6±10.7)次,支气管组织有炎性细胞浸润现象,血清过氧化氢含量增加(71.3±18.8)mol.L-1。而麻杏石甘汤高、中、低剂量组均能明显降低枸橼酸所致咳嗽次数,分别为(30.9±9.9),(39.8±8.6),(36.4±2.8)次(P<0.05),减少支气管黏膜下层细胞炎性细胞浸润,降低血清中过氧化氢含量,分别为(60.6±7.3),(44.7±10.1),(33.8±7.5)mol.L-1(P<0.05),但只有高剂量组才延长咳嗽潜伏期(P<0.05)。结论:麻杏石甘汤能防治枸橼酸所致豚鼠咳嗽,引起过氧化氢的改变可能是其作用机制之一。

The Effect of Maxing Shigan Decoction on Citric Acid-induced Cough in Guinea Pig

Objective: To investigate the effect of Maxing Shigan decoction on citric acid-induced cough in guinea pig. Method: Guinea pigs were randomly divided into six groups such as normal, model, codeine and three Maxing Shigan decoction groups (high-doses, middle-doses and low-doses). Drugs had been continuously administered for 7 days before citric acid-induced cough model in guinea pig was established. Frequency of cough and latent period were counted with the system of biomechanical parameter collection, which are responsible for cough signal, voice recording and counting. The content of serum H₂O₂ and catalase were detected using biochemical methods. Result: The frequency of cough (53. 6±10. 7), infiltration of inflammatory cells in bronchial submucosa and content of serum H₂O₂ (71. 3±18. 8) mol·L^-1 were increased in model groups. The frequency of cough, infiltration of inflammatory cells in bronchial submucosa and content of serum H₂O₂ were decreased by Maxing Shigan decoction (P<0. 05). But latent period of cough was only prolonged by high-doses Maxing Shigan decoction (P<0. 05). Conclusion: The citric acid-induced cough model in uinea g pig could be prevented and cured by Maxing Shigan decoction, and the changed H₂O₂may be one of mechanisms.