狐狸尾不同极性溶剂提取物体外抗氧化活性研究

目的:研究狐狸尾Uraria lagopodioides不同极性提取部位的体外抗氧化活性。方法:以乙酸乙酯、丙酮和95%乙醇为溶剂分别对狐狸尾进行冷浸提取,得到各种溶剂提取物EAE,AE,EE,采用紫外分光光度法分别测定总黄酮含量,并将各提取物配制成0.2～1.2 g.L-1,采用ABTS.+法、DPPH.法和铁离子还原能力法测定不同浓度提取物抗氧化活性。结果:测得狐狸尾EAE,AE,EE总黄酮含量分别为74.0,65.7,84.2 g.kg-1;狐狸尾各提取部位具有较强ABTS.+,DPPH.清除能力及还原能力,其中乙醇提取部位(EE)具有优异的抗氧化能力,在3个体系中,其半清除浓度(IC50)分别为0.52,1.36,4.88 g.L-1。结论:狐狸尾各提取物具有作为天然抗氧化剂和功能性食品进行研究和开发的潜力。

Antioxidant Activity of Different Extracts of Uraria lagopodioides

Objective: To study the antioxidant activity of different extracts of Uraria lagopodioides. Method: The total flavonoids content of ethyl acetate extract, acetone extract and 95% ethanol extract from U. lagopodioides by UV methods. Their antioxidant activities were investigated in present study with different doses (0. 2-1. 2 g·L^-1), employing various methods established in vitro systems, such as the scavenging activities on ABTS⁺ radical and DPPH radical, and reducing power. Result: The total flavonoids content of EAE, AE and EE was 74. 0, 65. 7, 84. 2 g·kg^-1, respectively. These plants possessed good scavenging activities on ABTS·⁺radical, DPPH· radical and reducing power activities, and the most outstanding one was found to be the ethanol extract, the IC₅₀ value of EE in these three essays was 0. 52, 1. 36, 4. 88 g·L^-1, respectively. Conclusion: This study showed that all the extracts could be employed as natural antioxidants and health care products.