疫苗免疫对小鼠神经系统发育影响的初步探讨

目的 探讨疫苗免疫对于小鼠神经系统发育的影响。 方法 将制备计划免疫9 种疫苗病原体的蛋白氨基酸序列与可能导致神经系统发育障碍的分子氨基酸序列进行比对，找到同源序列（ ≥ 5-6AA） 并进行化学合成，免疫乳鼠，筛选出可能导致神经系统发育障碍的氨基酸序列。对神经系统发育障碍血清样本进行ELISA 检测。 结果 神经系统发育障碍血清中抗AS-4 抗体滴度明显升高，与神经系统发育障碍相关分子氨基酸同源序列的AS-4 具有更大的研究价值。用AS-4 免疫乳鼠，在小鼠脑组织内可发现明显的T 淋巴细胞侵润。 结论 由于疫苗诱导，小鼠机体免疫系统对与疫苗相同序列的蛋白产生免疫反应。

Effect of vaccination on development of nervous system in mice

Objective To study the effect of vaccination on development of nervous system in mice. Methods Amino acid sequences in 9 vaccine pathogens were compared with those that may lead to developmental disturbance of the nervous system in mice. Homologous sequences（ ≥ 5-6AA） were synthesized to immunize the mice. Amino acid sequences that may lead to developmental disturbance of the nervous system were detected by ELISA. Results The serum anti-AS-4 antibody titer was significantly higher in mice with developmental disturbance of the nervous system than in those without developmental disturbance of the nervous system. The AS-4 with the amino acid homologous sequences in relation with the developmental disturbance of the nervous system was of greater research value. The infiltration of T lymphocytes was detected in brain tissue of AS-4 immunized mice. Conclusion The immune system of mice has immune response to the protein with homologous sequences of vaccine.