| CRM1在创伤性脑损伤大鼠脑中的表达变化 |
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| 引用本文： | 吴新民. CRM1在创伤性脑损伤大鼠脑中的表达变化[J]. 中国交通医学杂志, 2012, 26(6) |
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| 作者姓名： | 吴新民 |
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| 作者单位： | 吴新民 (南通大学附属医院神经内科,江苏,226001); |
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| 摘 要： | 目的:研究靶向调控运输蛋白(Chromosome region maintenance1,CRM1)在创伤性脑损伤中的表达变化。方法:采用Western blot和免疫组织化学荧光法检测脑损伤后CRM1在脑中的表达变化。结果:(1)假手术组皮质CRM1蛋白水平低,在损伤后12小时逐步增加,在损伤后3天达到高峰,之后恢复到正常水平。非手术对侧皮层中CRM1表达没有明显改变。(2)免疫组化荧光测定:损伤后CRM1表达水平不但上升,且有入核的亚定位变化,主要定位在神经元中。而假手术组和对侧则主要定位在胞浆中。结论:推测CRM1在创伤性脑损伤后与神经元凋亡有关。
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| 关 键 词： | 创伤性脑外伤 大鼠 免疫组织化学荧光法 Bradford测定法 靶向调控运输蛋白(CRM1) |
Expression Changes of CRM1 after traumatic brain injury in adult rats |
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| Abstract: | Objective:To study the CRM1 expression changes in traumatic brain injury(TBI).Methods: Western blot and immunohistochemical fluorescence were used to detect the CRM1 expression in the brain after brain injury.Results: After TBI,CRM1 protein expression levels increased in the ipsilateral side of the brain,while the contralateral expression showed to significant change.Conclusion: For the first time,reported CRM1 expression in the brain tissue of traumatic brain injury and it was speculated that the CRM1was associated with neuronal apoptosis in traumatic brain injury. |
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| Keywords: | CRM1 traumatic brain injury apoptosis rat |
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