| 野生型p53基因脂质体转染细胞疫苗的制备 |
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| 引用本文： | 孔祥波,谷新权,马庆杰. 野生型p53基因脂质体转染细胞疫苗的制备[J]. 中国免疫学杂志, 2000, 16(8): 426-427 |
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| 作者姓名： | 孔祥波 谷新权 马庆杰 |
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| 作者单位： | 1. 白求恩医科大学第三临床医学院泌尿外科,长春,130021
2. 白求恩医科大学第三临床医学院核医学科,长春,130021 |
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| 摘 要： | 目的：制备具有治疗活性的野生型Ｐ５３基因真核表达载体的细胞疫苗。方法：利用ＲＴ－ＰＣＲ从人外周血淋总ｍＲＮＡ中扩增出全长的野生型Ｐ５３基因,将其克隆入ＴＡ克隆载体的经扩增后,将ｐ５３ｃＤＮＡ连入ＰＣＭＶ真核表达载体中,利用ｌｉｐｏｆｅｃｔａｍｉｎｅ将其转染ＤＵ１４５细胞并通过免疫组化的方法检测其表达情况。结果：Ｐ５３基因可在ＤＵ１４５细胞中稳定表达。结论：制备了可稳定表达可ＤＵ１４５细胞中的野生型
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| 关 键 词： | 野生型p53基因 核酸疫苗 脂质体 前列腺癌 |
Construction of wide-type p53 gene vaccine |
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| KONG Xiang-Bo,GU Xin-Quan,MA Qing-Jie. Construction of wide-type p53 gene vaccine[J]. Chinese Journal of Immunology, 2000, 16(8): 426-427 |
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| Authors: | KONG Xiang-Bo GU Xin-Quan MA Qing-Jie |
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| Abstract: | Objective:To construct a wide type p53 gene vaccine which has the therapeutic activity.Methods:We get the intact wide type p53 gene from human peripheral lymphocyte total mRNA using RT PCR,then cloned it to TA vector and link to pCMV after gene amplification.pCMV p53 expression vectors were transfected in DU145 using lipofectamine and the protein was detected by immunohistochemistry.Results:p53 gene can express stability in DU145.Conclusion:We constructed a p53 gene vaccine what can express stability in Du145 cell line. |
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| Keywords: | Wide type p53 gene DU145 Gene vaccine |
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