人参不同部位中人参皂苷成份的分析

目的：建立UV/HPLC 法测定人参不同部位中人参皂苷含量。方法：通过对市场上通行的UV法和HPLC 法测得含量进行近似折算比较，以及对根部提取物与茎叶提取物中各人参皂苷成分HPLC 出峰比例的分析，建立鉴别不同来源人参皂苷的方法。结果：人参根部提取物中，Re颐Rb1颐Rc颐Rd 皂苷比例近似为1:2.20:0.94:0.97；人参茎叶中，该比例近似为1:（0.05~0.07）:（0.10~0.16）:（0.32~0.37）；对比掺混后的人参根部和人参茎叶提取物，Re:Rb1:Rc:Rd 理论值在上述两比例之间。结论：该方法简易、稳定、可靠，可作为人参根部和茎叶提取物中人参皂苷的鉴别方法。

Composition Analysis of Ginsenoside from Different Fractions of Ginseng

This study was aimed to measure the content of ginsenoside in ginseng extract by UV/HPLC. Through the approximate translation comparison of two different testing results with UV and HPLC in current market, and several typical HPLC peaks ratio analysis on contents of different ginsenoside extracted from the root, stem and leaves of ginseng, in order to establish identification method of ginsenoside from different sources. The results showed that the ratio for Re:Rb1:Rc:Rd in ginseng root extract was approximately 1:2.20:0.94:0.97. And the ratio for Re:Rb1:Rc:Rd in ginseng leave extract was approximately 1:(0.05~0.07):(0.10~0.16):(0.32~0.37). Compared with the mixture of ginseng root and ginseng leave extract, the theoretical value of Re:Rb1:Rc:Rd should between those two ratios. It was concluded that this method was simple, stable and reliable, which can be used as the identification method of ginsenoside from ginseng root, stem and leaves.