常用中药注射剂致敏性研究

目的：研究中药注射剂的过敏反应，测定致敏动物血清免疫球蛋白（IgE）抗体含量，观察其与过敏反应是否具有相关性，为提高过敏反应实验预测的准确性提供实验依据。方法：选用多种中药注射剂进行被动皮肤过敏实验（PCA）实验和类过敏反应研究，用ELISA 法对致敏动物血清进行血清IgE 水平检测。结果：双黄连注射液（SHL）、清开灵注射液（QKL）、鱼腥草注射液（YXC）、血塞通注射液（XST）、葛根素注射液（GGS）、穿琥宁注射液（CHN）的PCA 实验结果均为阴性，刺五加注射液（CWJ）的PCA 实验结果为阳性。SHL、QKL、YXC、XST、GGS、CHN、CWJ 注射液组血清总IgE 水平与生理盐水组无明显差异；SHL、QKL、YXC、XST、GGS、CHN、CWJ 注射液均引起了豚鼠的类过敏症状。结论：SHL、QKL、YXC、XST、GGS、CHN、CWJ 均能引起豚鼠的类过敏反应，其过敏反应的发生与血清IgE 抗体水平无相关性。

Effect of Traditional Chinese Medicine Injections on Allergic Reactions

This study was aimed to investigate allergic reactions of traditional Chinese medicine (TCM) injections,and to determine contents of serum IgE in sensitized animals. The correlation between preceding contents in serum and allergic reactions may be found, in order to offer experimental evidences for advancing the accuracy of anticipation by allergic reactions. Passive cutaneous anaphylaxis (PCA) tests and anaphylactoid reactions were used in a variety of TCM injections. ELISA method was used to determine the content of total serum IgE in sensitized animals.The PCA results of SHL, QKL, YXC, XST, GGS and CHN were negative. The PCA result of CWJ was positive.There was no significant difference for total serum IgE level between the experimental group and the normal saline group in the group of SHL, QKL, YXC, XST, GGS, CHN and CWJ. All TCM injections caused anaphylactoid symptoms in guinea pigs. It was concluded that all TCM injections can cause allergic reactions in guinea pig. And the allergic reactions of TCM injections were not correlated with serum total IgE.